start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=9 article-no= start-page=3229 end-page=3243 dt-received= dt-revised= dt-accepted= dt-pub-year=1958 dt-pub=19580930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The Effect of Polysaccharides Extracted from the Calf Bone Marrow on the Bone-Marrow Tissue Culture Part 1. The Effect of Polysaccharides on the Bone-Marrow Tissue Culture of Normal Rabbits kn-title=骨髄抽出多糖類物質の骨髄組織培養に及ぼす影響 第1編 正常家兎骨髄組織培養に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=With the purpose to elucidate more clearly the actual mechanism of the hematopoietic function of polysaccharides extracted from the calf bone marrow, the author performed bone-marrow tissue culture with addition of polysaccharides at various concentrations, and studied the effects of these substances on the bone marrow. The results are as follows: 1. An appropriate amount of polysaccharides helps to promote the growth of the bone marrow tissue and the wandering velocity of pseudoeosinophils in bone marrow in the coverslip culture. 2. Even in the bone-marrow tissue culture in the fluid medium an appropriate amount of the polysaccharide brings about an increase in the erythrocyte count and hemoglobin content. 3. In other words, the polysaccharides extracted from the calf bone marrow possess the action as to stimulate directly the bone marrow parenchyma, thus promoting the hematopietic function of the marrow. 4. Consequently, the hematopoietic action of these substances is not solely dependent upon the dilatation of the blood vessels in the bone-marrow but also on the promotion of the function of bone marrow parenchyma. en-copyright= kn-copyright= en-aut-name=MiyashitaKenji en-aut-sei=Miyashita en-aut-mei=Kenji kn-aut-name=宮下謙二 kn-aut-sei=宮下 kn-aut-mei=謙二 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科教室 END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=9 article-no= start-page=3245 end-page=3259 dt-received= dt-revised= dt-accepted= dt-pub-year=1958 dt-pub=19580930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The Effect of Polysaccharides Extracted from the Calf Bone Marrow on the Bone-Marrow Tissue Culture Part 2. The Effect of Polysaccharides with addition of Sera from Anemic Patients and Bone Marrow Inhibitory Agents on the Bone Marrow Tissue Culture of Rabbits kn-title=骨髄抽出多糖類物質の骨髄組織培養に及ぼす影響 第2編 貧血患者血清並びに骨髄機能抑制薬物添加家兎骨髄組織培養に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The author studied the effects of the addition of the polysaccharides extracted from the bone marrow in combination with sera of various anemic patients and inhibitory agents of bone marrow on the bone-marrow tissue cultura both in fluid medium and in cover-slip method; and obtained the following results: 1. The acceleration of the bone marrow function by polysaccharides extracted from the bone marrow acts antagonistically the action of the anemia inducing factors in sera of aplastic anemia, Banti's disease, and chronic myelogenous leukemia, and in cases it reactivates the bone-marrow function above that of the normal. 2. Even in the case of combination with Carzinophilin or nitromine, such an accelerative action of polysaccharides, antagonistically against the action of these inhibitory agents on the bone marrow, reactivates the bone-marrow function, but in this instance the antagonistic power is relatively less than that in the case of the above. en-copyright= kn-copyright= en-aut-name=MiyashitaKenji en-aut-sei=Miyashita en-aut-mei=Kenji kn-aut-name=宮下謙二 kn-aut-sei=宮下 kn-aut-mei=謙二 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科教室 END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=9 article-no= start-page=3261 end-page=3272 dt-received= dt-revised= dt-accepted= dt-pub-year=1958 dt-pub=19580930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The Effect of Polysaccharides Extracted from the Calf Bone Marrow on the Bone-Marrow Tissue Culture Part 3. The Effect of Polysaccharides on the Bone-Marrow Tissue Culture of Rabbits with Experimental Bone-Marrow Dysfunction induced by kolargol injection and X-ray irradiation and of Patients with Aplastic Anemia kn-title=骨髄抽出多糖類物質の骨髄組織培養に及ぼす影響 第3編 コラルゴール貧血家兎, X線障碍家兎並に再生不良性貧血患者骨髄組織培養に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=With the purpose to elucidate the mechanism of recuperating power of polysaccharides extracted from the bone marrow aoting on anemia, the author conducted a series of bone-marrow tissue culture of rabbits with experimental bone-marrow dysfunction and of patients with aplastic anemia, adding the polysaccharides to the culture medium; and obtained the following results: 1. In the bone-marrow tissue culture of rabbits with kolargol-anemia the addition of the polysaccharides accelerates the hone marrow function. 2. In the bone-marrow tissue culture of rabbits with acute bone-marrow dysfunction by X-ray irradiation the addition of such polysaccharides gives benefitial effects on the bone marrow function. 3. In the case of bone-marrow tissue culture of patients with aplastic anemia the addition of such polysaccharides promotes the tissue growth, the wandering velocity of cells, and induces an increase in the hemoglobin content and erythrocyte count. 4. However, in the above-mentioned experiments if the bone marrow function is extremely disturbed, the addition of these substances can not reactivate the lost function. 5. In other words, polysaceharides extracted from the bone marrow act directly as to stimulate the disturbed bone marrow so as to reactivate the hematopoietic function so long as the degree of the dysfunction still retains some possibility of recovery, but these substances can not reactivate the function of the bone marrow in the case where the function is so highly disturbed as to be completely obliterated. en-copyright= kn-copyright= en-aut-name=MiyashitaKenji en-aut-sei=Miyashita en-aut-mei=Kenji kn-aut-name=宮下謙二 kn-aut-sei=宮下 kn-aut-mei=謙二 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科教室 END