岡山医学会 Acta Medica Okayama 0030-1558 70 9 1958 骨髄抽出多糖類物質の骨髄組織培養に及ぼす影響 第1編 正常家兎骨髄組織培養に及ぼす影響 3229 3243 EN Kenji Miyashita With the purpose to elucidate more clearly the actual mechanism of the hematopoietic function of polysaccharides extracted from the calf bone marrow, the author performed bone-marrow tissue culture with addition of polysaccharides at various concentrations, and studied the effects of these substances on the bone marrow. The results are as follows: 1. An appropriate amount of polysaccharides helps to promote the growth of the bone marrow tissue and the wandering velocity of pseudoeosinophils in bone marrow in the coverslip culture. 2. Even in the bone-marrow tissue culture in the fluid medium an appropriate amount of the polysaccharide brings about an increase in the erythrocyte count and hemoglobin content. 3. In other words, the polysaccharides extracted from the calf bone marrow possess the action as to stimulate directly the bone marrow parenchyma, thus promoting the hematopietic function of the marrow. 4. Consequently, the hematopoietic action of these substances is not solely dependent upon the dilatation of the blood vessels in the bone-marrow but also on the promotion of the function of bone marrow parenchyma. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 70 9 1958 骨髄抽出多糖類物質の骨髄組織培養に及ぼす影響 第2編 貧血患者血清並びに骨髄機能抑制薬物添加家兎骨髄組織培養に及ぼす影響 3245 3259 EN Kenji Miyashita The author studied the effects of the addition of the polysaccharides extracted from the bone marrow in combination with sera of various anemic patients and inhibitory agents of bone marrow on the bone-marrow tissue cultura both in fluid medium and in cover-slip method; and obtained the following results: 1. The acceleration of the bone marrow function by polysaccharides extracted from the bone marrow acts antagonistically the action of the anemia inducing factors in sera of aplastic anemia, Banti's disease, and chronic myelogenous leukemia, and in cases it reactivates the bone-marrow function above that of the normal. 2. Even in the case of combination with Carzinophilin or nitromine, such an accelerative action of polysaccharides, antagonistically against the action of these inhibitory agents on the bone marrow, reactivates the bone-marrow function, but in this instance the antagonistic power is relatively less than that in the case of the above. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 70 9 1958 骨髄抽出多糖類物質の骨髄組織培養に及ぼす影響 第3編 コラルゴール貧血家兎, X線障碍家兎並に再生不良性貧血患者骨髄組織培養に及ぼす影響 3261 3272 EN Kenji Miyashita With the purpose to elucidate the mechanism of recuperating power of polysaccharides extracted from the bone marrow aoting on anemia, the author conducted a series of bone-marrow tissue culture of rabbits with experimental bone-marrow dysfunction and of patients with aplastic anemia, adding the polysaccharides to the culture medium; and obtained the following results: 1. In the bone-marrow tissue culture of rabbits with kolargol-anemia the addition of the polysaccharides accelerates the hone marrow function. 2. In the bone-marrow tissue culture of rabbits with acute bone-marrow dysfunction by X-ray irradiation the addition of such polysaccharides gives benefitial effects on the bone marrow function. 3. In the case of bone-marrow tissue culture of patients with aplastic anemia the addition of such polysaccharides promotes the tissue growth, the wandering velocity of cells, and induces an increase in the hemoglobin content and erythrocyte count. 4. However, in the above-mentioned experiments if the bone marrow function is extremely disturbed, the addition of these substances can not reactivate the lost function. 5. In other words, polysaceharides extracted from the bone marrow act directly as to stimulate the disturbed bone marrow so as to reactivate the hematopoietic function so long as the degree of the dysfunction still retains some possibility of recovery, but these substances can not reactivate the function of the bone marrow in the case where the function is so highly disturbed as to be completely obliterated. No potential conflict of interest relevant to this article was reported.