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ID 17137
Eprint ID
17137
フルテキストURL
Thumnail 89_667.pdf 1.17 MB
タイトル(別表記)
A new rapid assay method of collagenase activity and its clinical applications Part 1. A rapid assay method of collagenase activity using (14)C-labeled soluble collagen as substrate
著者
寺戸 国昭 岡山大学医学部第3内科教室
抄録
A rapid assay method of collagenase activity has been developed using (14)C-labeled soluble collagen as substrate. This method is based on following three parts; first, carrying out the enzyme reaction with (14)C-labeled collagen in solution containing 0.25M glucose to prevent collagen fiblil formation at 35℃ under neutral condition, second, denaturation of the enzyme digestion products at 35℃ for 1hr after stopping the reaction by adding o-phenanthroline and third, selective extraction of the denatured (14)C-products into dioxane at a final concentration of 50 % and counting (14)C-activity in the surpernatant. The rate of the reaction was about 10 times higher than that obtained by the conventional method using reconstituted collagen fiblils as substrate and relationship between enzyme activity and enzyme concentration was linear over a wider range regardless of the purity of collagenase.
発行日
1977-06-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
89巻
5-6号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
667
終了ページ
677
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/89/5-6/89_5-6_667/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
OAI-PMH Set
岡山大学
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Sort Key
13
Eprints Journal Name
joma