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ID 17139
Eprint ID
17139
フルテキストURL
Thumnail 89_679.pdf 1.19 MB
タイトル(別表記)
A new rapid assay method of collagenase activity and its clinical applications Part 2. Determination of collagenase activity in human leucocytes and synovial fluids by the newly developed method
著者
寺戸 国昭 岡山大学医学部第3内科教室
抄録
A newly developed collagenase assay method using (14)C-labeled soluble collagen as substrate has been applied to the determination of collagenase activity in human leucocytes and synovial fluids. The results indicated that collagenase activity in the both samples could be measured by this method as observed with tadpole collagenase, except that 35 % dioxane was preferable for the extraction of the enzyme digestion products in the case of synovial fluids. Collagenase activity determined by the present method was well correlated with that obtained by the conventional assay method using reconstituted collagen fiblils as substrate (r=0.963 and 0.829 (p<0.01) respectively). In preliminary experiments with leucocytes from patients with diabetes mellitus (DM), systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA), it was observed that collagenase activity in leucocytes increased significantly in SLE and from some patients with RA, while no appreciable differences were observed in diabetics, compared to that in normals. In synovial fluids from patients with RA and osteoarthritis (OA), the accumulation of large amount of inactive (latent) form of collagenase was demonstrated by the treatment of synovial fluids with 3M KI. And it was varied significantly in one patient at different time while no appreciable amount of the active form was observed during the period.
発行日
1977-06-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
89巻
5-6号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
679
終了ページ
691
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/89/5-6/89_5-6_679/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
OAI-PMH Set
岡山大学
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Sort Key
14
Eprints Journal Name
joma