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ID 57849
フルテキストURL
著者
Wei, Heng Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Wang, Chen Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Guo, Rui Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Takahashi, Ken Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Naruse, Keiji Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
抄録
Ischemic heart disease remains the largest cause of death worldwide. Accordingly, many researchers have sought curative options, often using laboratory animal models such as rodents. However, the physiology of the human heart differs significantly from that of the rodent heart. In this study, we developed a model of ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells (hiPS-CMs). After optimizing the conditions of ischemia, including the concentration of oxygen and duration of application, we evaluated the consequent damage to hiPS-CMs. Notably, exposure to 2% oxygen, 0 mg/ml glucose, and 0% fetal bovine serum increased the percentage of nuclei stained with propidium iodide, an indicator of membrane damage, and decreased cellular viability. These conditions also decreased the contractility of hiPS-CMs. Furthermore, ischemic conditioning increased the mRNA expression of IL-8, consistent with observed conditions in the in vivo heart. Taken together, these findings suggest that our hiPS-CM-based model can provide a useful platform for human ischemic heart disease research.
キーワード
Cardiomyocytes
Human induced pluripotent stem cells
Ischemic heart disease
Myocardial infarction
発行日
2019-12-10
出版物タイトル
Biochemical and Biophysical Research Communications
520巻
3号
出版者
Academic Press
開始ページ
600
終了ページ
605
ISSN
0006291X
NCID
AA00564395
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© 2019 The Authors.
論文のバージョン
publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1016/j.bbrc.2019.09.119
ライセンス
http://creativecommons.org/licenses/by-nc-nd/4.0/
Citation
Heng Wei, Chen Wang, Rui Guo, Ken Takahashi, Keiji Naruse, Development of a model of ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells, Biochemical and Biophysical Research Communications, Volume 520, Issue 3, 2019, Pages 600-605, ISSN 0006-291X, https://doi.org/10.1016/j.bbrc.2019.09.119.
オープンアクセス(出版社)
OA
オープンアーカイブ(出版社)
非OpenArchive