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ID 13634
Eprint ID
13634
フルテキストURL
Thumnail 113_33.pdf 3.77 MB
タイトル(別表記)
Establishment and characterization of cell line from ferric nitrilotriacetate (Fe-NTA)-induced rat renal cell carcinoma
著者
掛橋 千賀子 岡山県立大学保健福祉学部
森 將晏 岡山県立大学保健福祉学部
川端 晃幸 岡山大学医学部第一病理学教室
岡田 茂 岡山大学医学部第一病理学教室
抄録
The characteristics of ferric nitrilotriacetate (Fe-NTA)-induced rat renal cell carcinoma by intraperitoneal administration and the effects of hormones on the tumor have been studied in vivo. However, to our knowledge there have been no reports on the characteristics of the tumor cells. In this study, we established a tumor cell line from Fe-NTA induced rat renal tumour and investigated tumor cell characteristics. The tumor cell line RKFe was established from Fe-NTA induced rat renal tumor by collagenase digestion. RKFe showed epithelial morphology in culture, and had microvilli and basement membranes under electron microscopy. RKFe cells acquired tolerance to cytotoxicity of Fe-NTA. They also grew in soft agar culture and formed tumors by the inoculation of cells into nude mice. Histologically, transplanted tumors showed poorly defferentiated adenocarcinoma with several tubular structures. A subclone of RKFe, named RKFe-C6, showed metastasis to lungs. The RKFe cell line may be useful for studying the characteristics of Fe-NTA induced rat tumor cells.
キーワード
Fe-NTA
腎癌
培養細胞株の樹立
備考
原著
発行日
2001-04-28
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
113巻
1号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
33
終了ページ
39
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/113/1/113_1_33/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma