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ID 32952
フルテキストURL
著者
Murakami, Jun Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Asaumi, Jun-ichi Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Kawai, Noriko Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Tsujigiwa, Hidetsugu Department of Oral Pathology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Yanagi, Yoshinobu Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Nagatsuka, Hitoshi Department of Oral Pathology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Inoue, Tetsuyoshi Department of Oral Microbiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Kokeguchi, Susumu Department of Oral Microbiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Kawasaki, Shoji Department of Radiological Technology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Kuroda, Masahiro Department of Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Tanaka, Noriaki Department of Surgical Oncology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Matsubara, Nagahide Department of Surgical Oncology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
Kishi, Kanji Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University Graduate Schools
抄録
We speculated whether or not the expression level of telomerase reverse transcriptase (hTERT) would be modulated by agents targeting epigenetics in oral cancer cell lines. Although hTERT is known to be targeted by epigenetic changes, it remains unclear how chemoagents targeting epigenetics work on hTERT transcription. In the present study, the epigenetic effects of histone deacetylase (HDAC) inhibitor FR901228 on hTERT transcription were analysed by RT-PCR in oral cancer cell lines. The mRNA expression of hTERT was upregulated after exposure to FR901228 in hTERT-negative Hep2 cells, even in the hTERT highly expressed SAS and KB cells. Moreover, co-treatment of protein synthesis inhibitor cycloheximide (CHX) resulted in the induction of hTERT transcription by FR901228. This suggests that the induction of hTERT by FR901228 requires de novo protein synthesis to some extent and is more likely a direct than an indirect effect on epigenetic changes such as histone acetylation / deacetylation. We further examined the effect of FR901228 on c-myc protein, which is one of the main hTERT transcription activators. FR901228 repressed c-myc protein only in the absence of CHX, dependent of the enhancement of de novo protein synthesis. Our results indicate that c-myc protein is repressed indirectly by FR901228 but may not contribute FR901228-induced hTERT transcription. The present study showed that the HDAC inhibitor FR901228 induced the hTERT gene by a complex mechanism that involved other transcription factors except for c-myc, in addition to the inhibition of histone deacetylation.
キーワード
hTERT
FR901228
oral cancer
HDAC inhibitor
備考
Digital Object Identifer:10.1007/10.1007/s00280-004-0976-x
Published with permission from the copyright holder. This is the author's copy, as published in Cancer Chemotherapy and Pharmacology, July 2005, Volume 56, Issue 1, Pages 22-28.
Publisher URL:http://dx.doi.org/10.1007/s00280-004-0976-x
Direct access to Thomson Web of Science record
Copyright © 2005 Springer-Verlag. All rights reserved.
発行日
2005-07
出版物タイトル
Cancer Chemotherapy and Pharmacology
56巻
1号
出版者
Springer
開始ページ
22
終了ページ
28
ISSN
0344-5704
NCID
AA00598397
資料タイプ
学術雑誌論文
関連URL
author
言語
English
著作権者
Springer-Verlag
査読
有り
DOI
PubMed ID
Web of Sience KeyUT
Submission Path
dentistry_general/10