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ID 32071
JaLCDOI
フルテキストURL
著者
Takahashi, Fumio Okayama University
Kuramitsu, Makoto Okayama University
Tokuda, Masaaki Okayama University
Matsui, Hideki Okayama University
Itano, Toshifumi Okayama University
Murakami, Tetsu-Hide Okayama University
Hatase, Osamu Okayama University
Nishida, Isamu Okayama University
抄録

Cellular stimulating factors on cell proliferation in the supernatants of chick embryo carcases and adult muscles were studied. There were plural stimulating factors in embryonic and adult muscular supernatants that promoted cell proliferation without any supplement of sera and other materials. Salting-out methods with ammonium sulfate, ethanol fractionation, and isoelectric precipitation were used to isolate the stimulating factors, and these three methods proved the presence of plural stimulants on cell proliferation in the supernatants of chick embryo and adult muscles. The stimulants had altered physico-chemical properties and biological activities due to embryological development. The embryonic stimulants enhanced the synthesis of DNA and protein remarkably, and RNA synthesis in whole cell systems slightly. The muscular stimulants enhanced protein synthesis without any stimulation of DNA and RNA synthesis. Partial purification of the stimulants from the ethanol fractions was performed by DEAE-cellulose chromatography and Sephadex gel chromatography.

キーワード
chick growth factors
cell proliferation
growth regulation
DNA and RNA synthesis
protein synthesis
Amo Type
Article
発行日
1979-06
出版物タイトル
Acta Medica Okayama
33巻
3号
出版者
Okayama University Medical School
開始ページ
167
終了ページ
176
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID