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ID 13218
Eprint ID
13218
フルテキストURL
Thumnail 119_247.pdf 400 KB
タイトル(別表記)
Impaired coronary capillary hemodynamics with decreased glycocalyx thickness and irregular inner wall remodeling in right ventricle of pulmonary hypertensive rats
著者
梶谷 昌史 岡山大学大学院医歯薬学総合研究科 循環器内科学
抄録
We hypothesized that coronary capillary function is impaired with a decreased glycocalyx layer and uneven inner wall remodeling in right ventricles that are hypertrophied due to pulmonary hypertension (PH). Five-week-old male Sprague-Dawley rats were divided into 2 groups (PH: n=25, control: n=27). In the PH group, Monocrotaline (60 mg/kg SC) was administrated at 5 weeks. Coronary capillary hemodynamics was visualized in vivo at 8 weeks of age using our high-resolution intravital videomicrosopy, and RBC velocities in RV capillaries were evaluated, along with capillary diameters. Configuration of the capillary cast was assessed by a confocal laser scanning microscope. The thickness of the glycocalyx on the capillary endothelial surface was evaluated with Alcian blue 8GX by electron microscopy. RV systolic pressure increased in PH (by 142%, p<0.01). The diameters of capillaries were uneven in PH (coefficient of variation of diameters along capillary trees; 24±8% vs. 11±3%, p<0.05). The thickness of the glycocalyx in PH was less than half that of control (0.20±0.05 vs. 0.45±0.14 μm, p<0.05). RBC velocity was decreased in PH (820±110 vs. 1,100±230 μm/sec, p<0.05). The capillary flow reduction in PH may be closely related to the changes in the glycocalyx and vessel irregularity, resulting in insufficient oxygen supply to the heart.
キーワード
モノクロタリン (monocrotaline)
肺高血圧 (pulmonary hypertension)
冠毛細管 (coronary capillary)
グリコカリックス (glycocalyx)
リモデリング (remodeling)
備考
原著
発行日
2008-01-04
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
119巻
3号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
247
終了ページ
251
ISSN
00301558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
http://www.jstage.jst.go.jp/article/joma/119/3/119_247/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma