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ID 52277
フルテキストURL
著者
Kakeda, Katsuyuki Mie Univ, Grad Sch Bioresources
Ishihara, Norimitsu Mie Univ, Grad Sch Bioresources
Izumi, Yohei Okayama Univ, Inst Plant Sci & Resources
Sato, Kazuhiro Okayama Univ, Inst Plant Sci & Resources 科研費研究者番号
Taketa, Shin Okayama Univ, Inst Plant Sci & Resources
抄録
Most cereal crops have hulless grains (naked caryopses) with a free-threshing trait, whereas the majority of barley cultivars show hulled (covered) caryopses. The naked caryopsis in barley is genetically controlled by a single locus, nod. The Nud gene (the covered caryopsis allele) encodes an ethylene response factor (ERF) family transcription factor that regulates a lipid biosynthetic pathway. For functional analysis of the barley Nud gene, we produced transgenic rice expressing Nod in the developing caryopses. All transgenic lines had caryopses that were easily dehulled at maturity, indicating that the naked caryopsis phenotype remained in spite of expression of the Nod transgene. Histochemical and lipid analyses of the transgenic rice caryopses did not show increased lipid accumulation on the surface of developing caryopses, suggesting that the Nud-mediated lipid pathway may not function in rice caryopses. The predicted rice ortholog of Nod, Os06ERF was expressed specifically in the developing caryopses. However, expression of Os06ERF ceased at an earlier developmental stage than that of the native Nod gene in barley caryopses, which was also the case for expression of the Nod transgene. This raises the alternative hypothesis that the timing of Nod expression may be critical for activating the pathway for hull-caryopsis adhesion.
キーワード
Nud gene
transformation
covered/naked caryopsis
lipid biosynthesis
ERF/AP2
grass
発行日
2011-03
出版物タイトル
Breeding Science
61巻
1号
開始ページ
35
終了ページ
42
ISSN
1344-7610
資料タイプ
学術雑誌論文
言語
English
著作権者
Copyright © 2011 by JAPANESE SOCIETY OF BREEDING
論文のバージョン
publisher
査読
有り
DOI
Web of Sience KeyUT