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ID 40624
フルテキストURL
タイトル(別表記)
The effects of calmodulin inhibitors (W-7, TFP) in cultured glioma cells
著者
武家尾 拓司 岡山大学医学部脳神経外科学教室
抄録
The effects of calmodulin inhibitors [W-7: N-(6-aminohexyl)-5-chloro-1-naphthalensulfonamide-HCl, TFP: Trifluoperazine]; cell growth inhibition, morphologic changes, and alterations of flow cytometry DNA hisotgram were studied in cultured glioma cells. The cell lines used in this experiment were KC and KY, which had been established from human glioblastomas, and C-6 from a methylnitrosourea-induced rat glioma. A dose-dependent growth inhibition was found in cells exposed to calmodulin inhibitors (W-7, TFP). The morphologic changes of cells were manifested by a rounding of the cells with numerous fine electron dense granules in the cytoplasm as early as 3 hours following exposure to calmodulin inhibitors. The influence of calmodulin inhibitors on cell kinetics was analysed by flow cytometry which revealed an accumulation of cells in G(2)+M phases within 24 hours after treatment. These results, together with the previous reports, indicate that the effects of calmodulin inhibitors on cell growth may be due not only to the inhibition of DNA synthesis in the G(1)/S boundary phase but also to the inhibition of tubulin disassembly in G(2)+M phases.
キーワード
カルモデュリン阻害剤
培養グリオーマ細胞
細胞増殖抑制
DNAヒストグラム
発行日
1990-04
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
102巻
3-4号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
465
終了ページ
483
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/102/3-4/102_3-4_465/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
OAI-PMH Set
岡山大学
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Sort Key
17
Eprints Journal Name
joma