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ID 16567
Eprint ID
16567
フルテキストURL
Thumnail 93_489.pdf 895 KB
タイトル(別表記)
Studies on human peripheral monocyte chemotaxis Part I. A technical study
著者
片岡 幹男 岡山大学医学部第二内科教室
抄録
The human monocyte is an important member of the host defence system, but has been little studied in vitro. In this report, I describe a reproducible and simple method for monocyte chemotaxis in man, using a Boyden chamber. Monocyte suspension was prepared by Ficoll-Hypaque gradient sedimentation. The purity of the suspension was checked with Peroxidase-Giemsa staining. Chemotaxis assay was performed using a 5 μm pore sized Millipore filter(thickness:150 μm) and a Nuclepore filter (thickness:13 μm). When the Millipore filter was used, migrated cells stayed in the filter, while it appeared that cells detached from the filter when the Nuclepore filter was used. In the modified Boyden method using a Millipore filter, more than 3×10(5) monocytes had to be added to the upper room of the chamber, and the duration of 90 minutes was adequate for incubation. Zymosan activated human serum diluted 20 percent was used as the chemoattractant. To estimate chemotaxis in the Millipore filter, migrated cells in the filter were counted at 30 μm depth.
キーワード
monocyte
chemotaxis
Boyden chamber
Millipore filter
発行日
1981-06-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
93巻
5-6号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
489
終了ページ
498
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/93/5-6/93_5-6_489/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma