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ID 52405
JaLCDOI
フルテキストURL
Thumnail 68_2_89.pdf 755 KB
著者
Sako, Shinichi Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kariyama, Reiko Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Mitsuhata, Ritsuko Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Yamamoto, Masumi Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Wada, Koichiro Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ishii, Ayano Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Uehara, Shinya Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kokeguchi, Susumu Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kusano, Nobuchika Department of Laboratory Medicine, Okayama University Hospital
Kumon, Hiromi Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
抄録
We conducted a study on molecular epidemiology and clinical implications of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa isolated from urine. Over a 10-year period from 2001 through 2010, a total of 92 MBL-producing P. aeruginosa urine isolates were collected from patients (one isolate per patient) who were admitted to 5 hospitals in Okayama Prefecture, Japan. When cross-infection was suspected in the hospital, pulsed-field gel electrophoresis was performed. In the resulting dendrogram of 79 MBL-producing P. aeruginosa urine isolates, no identical isolates and 7 pairs of isolates with ≥80% similarity were found. The biofilm-forming capabilities of 92 MBL-producing P. aeruginosa urine isolates were significantly greater than those of 92 non-MBL-producing urine isolates in a medium of modified artificial urine. The imipenem resistance transferred in 16 of 18 isolates tested, and these frequencies were in the range of 10-3 to 10-9. All of 18 isolates tested belonged to internationally spread sequence type 235 and had 3 gene cassettes of antimicrobial resistance genes in the class 1 integron. The strong biofilm-forming capabilities of MBL-producing P. aeruginosa urine isolates could be seriously implicated in nosocomial infections. To prevent spread of the organism and transferable genes, effective strategies to inhibit biofilm formation in medical settings are needed.
キーワード
Pseudomonas aeruginosa
metallo-β-lactamase
molecular epidemiology
biofilm
urinary tract infection
Amo Type
Original Article
発行日
2014-04
出版物タイトル
Acta Medica Okayama
68巻
2号
出版者
Okayama University Medical School
開始ページ
89
終了ページ
99
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
著作権者
CopyrightⒸ 2014 by Okayama University Medical School
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/52504