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ID 34242
フルテキストURL
著者
Tahara, Makoto Okayama University
Aoki, Takahiro JEOL Limited
Suzuka, Shinya SK Foods Company, Limited
Yamashita, Hiroki Okayama University
Tanaka, Masaru National Agricultural Research Center
Matsunaga, Sachi Kabaya Foods Corporation
Kokumai, Shuhei Okayama University
抄録

Many plant retrotransposons have been characterized, but only three families (Tnt1, Tto1 and Tos17) have been demonstrated to be transpositionally competent. We followed a novel approach that enabled us to identify an active element of the Ty1-copia retrotransposon family with estimated 400 copies in the sweetpotato genome. DNA sequences of Ty1 -copia reverse transcriptase (RTase) from the sweetpotato genome were analyzed, and a group of retrotransposon copies probably formed by recent transposition events was further analyzed. 3’RACE on callus cDNA amplified transcripts containing long terminal repeats (LTR) of this group. The sequence -specific amplification polymorphism (S-SAP) patterns of the LTR sequence in the genomic DNA were compared between a normal plant and callus lines derived from it. A callus -specific S-SAP product was found into which the retrotransposon detected by the 3’RACE had been transposed apparently during cell culture. We conclude that our approach provides an effective way to identify active elements of retrotransposons with high copy numbers.

キーワード
retrotransposon
Ipomoea batatas
S-SAP
transposition
備考
Digital Object Identifer:10.1007/s00438-004-1044-2
Published with permission from the copyright holder. This is the institute's copy, as published in the Journal of Molecular Genetics and Genomics, August 2004, Volume 272, Issue 1, Pages 116-127.
Publisher URL:http://dx.doi.org/10.1007/s00438-004-1044-2
Direct access to Thomson Web of Science record
Copyright © 2004 Springer-Verlag. All rights reserved.
発行日
2004-8
出版物タイトル
Molecular Genetics and Genomics
272巻
1号
開始ページ
116
終了ページ
127
資料タイプ
学術雑誌論文
言語
English
査読
有り
DOI
Web of Sience KeyUT
Submission Path
biochemistry/4