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ID 19403
Eprint ID
19403
フルテキストURL
Thumnail 70_4643.pdf 406 KB
タイトル(別表記)
Studies on the Fixation of the Dyes used in Supravital Staining of Cells Part 2. Reaction Between Dyes and Several Fixatives
著者
真木 照雄 岡山大学医学部病理学教室
抄録
In the first paper the author reported that the dyes used generally for supravital staining form insoluble precipitates with the Nessler's reagent suggesting that this reagent may be used for the fixation of the dyes used in supravital staining of cells. However, some authors report that several fixatives those containing mercuric ion or picric acid can be used for the fixation of dyes used in supravital staining of cells. In this paper the author presents the results observed on the reactions of various dyes with several fixatives. Experiment proved that some fixatives containing Hg or picric acid form insoluble colored precipitates with dyes. The formation of the precipitates is most marked in Janus green B and less marked in Nile blue. By treating picric acid the dyes change their color to that with yellow tone. No precipitates can remain undissolubled when they are exposed to the organic solvents, like alcohol, aceton etc. Janus green B precipitates show some resistance to the action of organic solvents but less resistance to Nile blue. It follows then for the observation of the supravitally stained granules on fixed cells, organic solvents should be avoided in any process of fixation and dehydration.
発行日
1958-12-31
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
70巻
12号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
4643
終了ページ
4649
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/70/12/70_12_4643/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma