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ID 19405
Eprint ID
19405
タイトル(別表記)
Studies on the Fixation of the Dyes used in Supravital Staining of Cells Part 3. Observations on the Blood Cells Supravitally Stained and Fixed by the Various Fixatives
著者
真木 照雄 岡山大学
抄録(別表記)
On the basis of the chemical reactions of the dyes with various fixatives in vitro the author observed the blood cells stained supravitally by various dyes and fixed with several fixatives, which has been proved to form the water insoluble precipitates with the dyes. Many known fixatives containing mercury prove to have the ability to fix the dyes used in supravital staining of cells. but the cell structure becomes often indistinct with some insoluble side precipitates which are not easy to be removed without losing some of the fixed dyes and the dye showed the tendency to fade relatively in a short period of time. In the case of the fixatives containing picric acid the results ware almost the same and yet the supravitally stainel granules changed the original color to rather yellowish ones. Whereas the method established by the author, the application of the Nessler's method, proved to be the most excellent one. By this method most of the supravitally stained granules are fixed on the cells retaining their fine structure. The poet-staining of the nucleus can be made by hematoxylin. And yet the dyes can be retained for a fairly long period of 2-3 months, without losing or changing their original colors.
発行日
1958
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
70巻
12号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
4651
終了ページ
4657
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/70/12/70_12_4651/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma