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ID 19407
Eprint ID
19407
タイトル(別表記)
Studies on the Fixation of the Dyes used in Supravital Staining of Cells Part 4. Observations on the Various Blood Cells Supravitally Stained and Fixed by Potassium Mercuric Iodide
著者
真木 照雄 岡山大学
抄録(別表記)
By the methods reported in the previous papers the anther observed the supravitally stained granules on the nucleus stained with hematoxylin. As the results it has been revealed that in lymphocytes, monocytes, myelocytic cells and Yoshida sarcoma cells, Janus green B stains mitochondria selectively but not the other components. The irregular forms of mitochondria stained with Janus green B can be recongnized frequently. This seems to be the result of the structural demolition caused by the staining itself. But in some cells the number of mitochondria which are stained by this dye, is rather small compared with those found under the phase-contrast or the electron-microscopes. Then it is assumed that Janus green B stains mitochondria selectively but not all of them. In general neutral red seems not to stain Golgi apparatus selectively, and neutral red granules are found to be irregular, both in their distribution in cytoplasma and in their size, all the granules being almost round in shape. But in small cells like monocytes, sometimes in lymphocytes the granules tend to gather in the area of Golgi zone. This thendency is recongnized especially markedly in the cells from the lower animals In the rosette formation of the neutral red Yoshida sarcoma cells have almost the same picture as that of monocytes but their mitochondria stainable with Janus green B are larger in number compared with those of monocytes. Brilliant crecyl blue and Nile blue seem also to stain mitochondria, but some other components like endoplasmic reticulum, neutrophilic granules and others in leucocytes, which can be stained by these dyes as the stained granules are very large in number and show marked variety in their sizes.
発行日
1958
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
70巻
12号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
4659
終了ページ
4667
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/70/12/70_12_4659/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma