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ID 53953
フルテキストURL
著者
Tamura, Takashi Graduate School of Environmental and Life Science, Okayama University
Tsunekawa, Naoki Institute of Industrial Science, the University of Tokyo
Nemoto, Michiko Graduate School of Environmental and Life Science, Okayama University
Inagaki, Kenji Graduate School of Environmental and Life Science, Okayama University
Hirano, Toshiyuki Institute of Industrial Science, the University of Tokyo
Sato, Fumitoshi Institute of Industrial Science, the University of Tokyo
抄録
Oxygen tolerance of selenium-containing [NiFeSe] hydrogenases (Hases) is attributable to the high reducing power of the selenocysteine residue, which sustains the bimetallic Ni–Fe catalytic center in the large subunit. Genes encoding [NiFeSe] Hases are inherited by few sulphate-reducing δ-proteobacteria globally distributed under various anoxic conditions. Ancestral sequences of [NiFeSe] Hases were elucidated and their three-dimensional structures were recreated in silico using homology modelling and molecular dynamic simulation, which suggested that deep gas channels gradually developed in [NiFeSe] Hases under absolute anaerobic conditions, whereas the enzyme remained as a sealed edifice under environmental conditions of a higher oxygen exposure risk. The development of a gas cavity appears to be driven by non-synonymous mutations, which cause subtle conformational changes locally and distantly, even including highly conserved sequence regions.
備考
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発行日
2016-01-28
出版物タイトル
Scientific reports
6巻
出版者
Nature Publishing Group
開始ページ
19742
ISSN
2045-2322
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1038/srep19742
言語
English
OAI-PMH Set
岡山大学
著作権者
© 2016 Nature Publishing Group
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT