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ID 49559
フルテキストURL
著者
Kamiya, K Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci
Aoyama, Y Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci
Shirafuji, Y Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci
Hamada, T Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci
Morizane, S Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci 科研費研究者番号
Fujii, K Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci
Hisata, K Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci
Iwatsuki, K Okayama Univ, Dept Dermatol, Grad Sch Med Dent & Pharmaceut Sci
抄録
Background Antidesmoglein (anti-Dsg) 3 serum antibody titres are usually correlated with the disease activity of pemphigus vulgaris (PV), but some patients retain high titres even in remission. Objectives The aim of our study was to determine whether anti-Dsg3 antibodies in PV sera recognized calcium (Ca2+)-dependent or non-Ca2+-dependent epitopes, and to evaluate their pathogenicity. Methods Dsg3 baculoprotein-coated enzyme-linked immunosorbent assay (ELISA) plates were treated with 0.5 mmol L-1 ethylenediaminetetraacetic acid (EDTA). The binding ability of anti-Dsg3 monoclonal antibodies (mAbs) was analysed. Eight of the 83 patients with PV who were screened had elevated Dsg3 ELISA index values > 100 in remission. The binding ability of these PV sera was analysed. We evaluated the pathogenicity of anti-Dsg3 serum antibodies against the non-Ca2+-dependent epitopes using a dissociation assay. Results The reactivity of pathogenic anti-Dsg3 mAbs against the Ca2+-dependent epitopes diminished markedly in the EDTA-treated ELISA, whereas no such reduction was observed in mAbs against the non-Ca2+-dependent epitopes. The sera of all the patients contained antibodies against both Ca2+-dependent and non-Ca2+-dependent epitopes. In six out of the eight patients, the ratio of antibodies against Ca2+-dependent to non-Ca2+-dependent epitopes decreased in remission. EDTA-treated Dsg3 baculoproteins adsorbed anti-Dsg3 serum antibodies against the non-Ca2+-dependent epitopes, but the remnant PV antibodies retained the ability to induce acantholysis in the dissociation assay. Conclusions We have established an assay to measure indirectly the titres of anti-Dsg3 serum antibodies against the Ca2+-dependent epitopes, based on the differences between EDTA-untreated and EDTA-treated ELISA index values, as a routine laboratory test to reflect the pathogenic anti-Dsg3 serum antibody titres more accurately.
発行日
2012-08
出版物タイトル
British Journal of Dermatology
167巻
2号
開始ページ
252
終了ページ
261
ISSN
0007-0963
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1111/j.1365-2133.2012.10929.x
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/49132
言語
English
著作権者
© 2012 British Association of Dermatologists
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT