このエントリーをはてなブックマークに追加
ID 10573
Eprint ID
10573
フルテキストURL
タイトル(別表記)
逆転写酵素・DNAポリメラーゼを用いたRT-PCRによる増幅反応で確認されたペッパーマイルドモットルウイルス(PMMoV)感染ピーマンの果実種子におけるウイルスの効果的な不活性化方法
著者
豊田 和弘 岡山大学
Hikichi, Yasufumi Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
Takeuchi, Shigeharu Laboratory of Plant Pathology, Kochi Agricultural Research Center
Okumura, Ako Laboratory of Plant Pathology, Iwate Biotechnology Research Center (IBRC)
Nasu, Yoshiko Laboratory of Plant Pathology, Iwate Biotechnology Research Center (IBRC)
Okuno, Tetsuro Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
Suzuki, Kazumi Laboratory of Plant Pathology, Iwate Biotechnology Research Center (IBRC)
抄録
A method for efficient inactivation Papper mild mottle virus (PMMoV) in harvested seeds of green pepper was examined based on the infectivity on the leaves of Nicotina glutinosa L.(a local host) and a reverse transcription and polymerase chain reaction (RT-PCR)-based amplification of the viral RNA. The seed homogenates from PMMoV-infected plants produced a large number of necrotic local lesion in N.glutinosa, but soaking the seeds in 10% (w/v) tri-sodium phosphate (Na3PO4) for 20 min or dry sterilization (70C,3h) effectivity eliminated the abundance of PMMoV. However, no necrotic lesion on N.glutinosa or the RT-PCR-basaed amplification was observed with seeds that has been disinfected by Na3PO4 in combination with dry sterilization.
キーワード
Capsicum annuum L.
pepper mild mottle virus (PMMoV)
RT-PCR
seed disinfection
seed transmission
発行日
2004-02
出版物タイトル
岡山大学農学部学術報告
出版物タイトル(別表記)
Scientific reports of the Faculty of Agriculture, Okayama University
93巻
1号
出版者
岡山大学農学部
出版者(別表記)
Faculty of Agriculture,Okayama University
開始ページ
29
終了ページ
32
ISSN
0474-0254
NCID
AN00033029
資料タイプ
紀要論文
言語
English
論文のバージョン
publisher
査読
無し
Eprints Journal Name
srfa