岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 放射線照射家兎肝より抽出せる細胞毒のウニの受精及び卵割に与える影響について 1307 1311 EN Satimaru Seno Michio Yamamoto Kozo Utsumi Midori Shiwaku Eiko Akagi Yoshiko Iguchi Junichi Kobayashi Hisako Ueno The effects of the OX substance, which was extracted from the liver of irradiated rabbits, on the sperm and egg, unfertilized and fertilized eggs of sea urchin (Hemicentrotus pulcherrimus) have been observed. 1. The sperms exposed to OX showed a decreased mortility and lost the ability to stimul to the egg to raise up fertilizing membrane suggesting the inability of giving physical shock to the egg surface or the damage of acrosome. 2. The unfertilized eggs exposed to OX retain the ability of the formation of fertiliging membrane and cleavage but showed remarkable delay in both the length of time for the membrane formation and cleavage. 3. The most sensitive effects on the fertilized eggs have been observed on the initial stage of fusion of sperm and egg nuclei and the metaphase. Immediately after the fertilization in the egg exposed to OX the fusion of sperm and egg nuclei was inhibited and in the cells on metaphase the cleavage was delayed by the prolonged period of transition from metaphase to telophase. 4. From the results with the consideration of the effects of X-rays on cleavage appearing in the past report the authors concluded the OX will be the essential substance representing the effects of X-rays on the cell cleavage. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 放射線照射時に発生する溶血性物質に就いて 第二編 溶血性物質の定性的研究並びに生物作用に就いて 1561 1567 EN Eiko Akagi The cause of irradiation anemia seems to lie in biological phenomena as well as in hemolytic toxin produced by X-Ray irradiation. It has been confirmed that since phospholipid fraction in vivo shows strong toxicity in direct proportion to the dose of X-rays, the actual hemolytic substance is lysolecithin. This substance is proved to give rise to marked morphological changes in hematopoietic organs, and it has also been clarified that the same substance acting on sea-urchin egg brings about a delay in egg division. However the same snbstance does not induce any striking change in the respiratory enzyme system. No potential conflict of interest relevant to this article was reported.