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ID 308
Eprint ID
308
フルテキストURL
タイトル(別表記)
シクロヘキシミドのタバコ葉核酸代謝におよぼす影響
著者
大内 成志 岡山大学
古沢 巌 Department of Plant Science, University of Alberta
赤井 重恭 Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University
抄録
The effect of cycloheximide on RNA metabolism in tobacco leaf discs was analyzed to signify its inhibitive or stimulative effect on TMV multiplication. RNA content increased in the leaf discs incubated in 1.0 or 3.0 ppm solution. 32P-incorporation into RNA, however, was accelerated at 1.0 ppm and disturbed at 3.0 ppm, suggestirg th~t the increment of RNA in the leaf disc<- treated at 1. O ppm is qualitatively different from that in the discs incubated at 3.0 ppm, i. e., the former is the result of stimulated synthesis while the latter is mainly due to the inhibition of degradation. Column chromatograms with methylated albumin Kiselguhr clearly elucidated that incubation of discs in 3.0 ppm led to the abolition of the integrity of the light ribosomal RNA. The change in chromatographic pattern was discernible 24 hr after the initiation of treatment of leaf discs. Incubation in 1.0 ppm cycloheximide, however, did not give rise to a drastic change in chromatograms, although an undulate incorporation of 32P-orthophosphate into ribosomal RNAs was noticed. 14C-glycine incorporation into RNA suggested that cycloheximide not only enhances RNA synthesis but also stimulates the synthesis of purine bases. Ribonuclease activity of leaf homogenate remarkably decreased by cycloheximide treatment, suggesting that the accumulation of RNA in the discs treated at 3.0 ppm could be acounted for by the retardation in RNA degradation. The inhibitory and stimulative effects of cycloheximide on TMV multiplication were discussed on the basis of these results.
発行日
1972
出版物タイトル
岡山大学農学部学術報告
出版物タイトル(別表記)
Scientific Reports of the Faculty of Agriculture Okayama University
40巻
1号
出版者
岡山大学農学部
出版者(別表記)
Faculty of Agriculture, Okayama University
開始ページ
9
終了ページ
18
ISSN
0474-0254
NCID
AN00033029
資料タイプ
紀要論文
言語
English
論文のバージョン
publisher
査読
無し
Eprints Journal Name
srfa