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ID 18759
Eprint ID
18759
フルテキストURL
Thumnail 70_123.pdf 3.13 MB
タイトル(別表記)
Analytical Studies on the Colorimetric Estimation of the Activity of Succinic Dehydrogenase System with Neotetrazolium Chloride
著者
小田 琢三 岡山大学医学部病理学教室
関 周司 岡山大学医学部病理学教室
柴田 高志 岡山大学医学部病理学教室
酒井 晃 岡山大学医学部病理学教室
岡崎 博明 岡山大学医学部病理学教室
抄録
Despite the excellence in bringing about reactions, there are still many difficulties and discrepancies in the use of neotetrazolium chloride for measuring the activity of succinic dehydrogenase system of tissue homogenate. Therefore, with the purpose to find out a measuring method simpler and more accurate, the authors performed some experimets, first on the enzymic reactions of tissue homogenate and then after stopping these reactions by using various agents, tested the ease with which reduction products are extracted with various solvents, and finally compared the absorption spectra of these liquid extracts with those of the products reduced chemically by sodium hydrosulfite. As the results, the extraction of the reduction products with acetone-ether (mixed in equal proprtion v/v) after stopping the reaction with 10 per cent formalin solution was found to be the simplest and best method, and moreover, the absorption spectra of extracts of the products obtained by enzymatical or chemical reduction proved to he uniform and the both of which showed the maximum absorption at the wave length of 520 mμ. In addition, the concentrations of sodium succinate and neotetrazolium chloride, the content and kinds of tissues, pH values and concentration of phosphate buffer solutions, the duration of reaction, aerobic or anaerobic conditions, various factors either enhancing or in hibitory to reaction, were all regulated absolutely or relatively; and by drawing absorption curves of the reduction products, these enzymatic reactions were analyzed and scrutinyzed Then the authors established a method which is general, simple and accurate in measuring the enzymatic activities of a small quantity of fresh tissues in contrast with those of hist chemically stained specimens.
発行日
1958-01-31
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
70巻
1号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
123
終了ページ
135
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/70/1/70_1_123/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma