JBiotechnology_185_57–62.pdf 1.21 MB
Monden, Yuki Graduate School of Environmental and Life Science, Okayama University
Takasaki, Kazuto FASMAC Co., Ltd.
Futo, Satoshi FASMAC Co., Ltd.
Niwa, Kousuke Graduate School of Biomedical Engineering, Tohoku University
Kawase, Mitsuo Graduate School of Biomedical Engineering, Tohoku University
Akitake, Hiroto Graduate School of Environmental and Life Science, Okayama University
Tahara, Makoto Graduate School of Environmental and Life Science, Okayama University
In many crops species, the development of a rapid and precise cultivar discrimination system has been required for plant breeding and patent protection of plant cultivars and agricultural products. Here, we successfully evaluated strawberry cultivars via a novel method, namely, the single tag hybridization (STH) chromatographic printed array strip (PAS) using the PCR products of eight genomic regions. In a previous study, we showed that genotyping of eight genomic regions derived from FaRE1 retrotransposon insertion site enabled to discriminate 32 strawberry cultivars precisely, however, this method required agarose/acrylamide gel electrophoresis, thus has the difficulty for practical application. In contrast, novel DNA detection method in this study has some great advantages over standard DNA detection methods, including agarose/acrylamide gel electrophoresis, because it produces signals for DNA detection with dramatically higher sensitivity in a shorter time without any preparation or staining of a gel. Moreover, this method enables the visualization of multiplex signals simultaneously in a single reaction using several independent amplification products. We expect that this novel method will become a rapid and convenient cultivar screening assay for practical purposes, and will be widely applied to various situations, including laboratory research, and on-site inspection of plant cultivars and agricultural products.
Journal of Biotechnology
© 2014 The Authors. Published by Elsevier B.V.