フルテキストURL fulltext.pdf
著者 Katsui, Kuniaki| Ogata, Takeshi| Watanabe, Kenta| Katayama, Norihisa| Kuroda, Masahiro| Kiura, Katsuyuki| Hiraki, Takao| Maeda, Yoshinobu| Toyooka, Shinichi| Kanazawa, Susumu|
キーワード cisplatin docetaxel dose-volume histogram non-small cell lung cancer PACIFIC trial radiation pneumonitis
発行日 2020-05-04
出版物タイトル Cancer Medicine
出版者 Wiley
ISSN 2045-7634
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 © 2020 The Authors.
論文のバージョン publisher
PubMed ID 32364685
DOI 10.1002/cam4.3093
Web of Science KeyUT 000530411600001
関連URL isVersionOf https://doi.org/10.1002/cam4.3093
著者 Murakami, Jun| Asaumi, Jun-ichi| Maki, Yuu| Tsujigiwa, Hidetsugu| Kuroda, Masahiro| Nagai, Noriyuki| Yanagi, Yoshinobu| Inoue, Tetsuyoshi| Kawasaki, Shoji| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji|
発行日 2004-2
出版物タイトル Oral Oncology
40巻
6号
資料タイプ 学術雑誌論文
著者 Murakami, Jun| Asaumi, Jun-ichi| Kawai, Noriko| Tsujigiwa, Hidetsugu| Yanagi, Yoshinobu| Nagatsuka, Hitoshi| Inoue, Tetsuyoshi| Kokeguchi, Susumu| Kawasaki, Shoji| Kuroda, Masahiro| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji|
発行日 2005-07
出版物タイトル Cancer Chemotherapy and Pharmacology
56巻
1号
資料タイプ 学術雑誌論文
フルテキストURL MMR20_3_2963.pdf
著者 Yoshimura, Yuuki| Kuroda, Masahiro| Sugianto, Irfan| Khasawneh, Abdullah| Bamgbose, Babatunde O.| Hamada, Kentaro| Barham, Majd| Tekiki, Nouha| Kurozumi, Akira| Matsushita, Toshi| Ohno, Seiichiro| Kanazawa, Susumu| Asaumi, Junichi|
キーワード magnetic resonance imaging apparent diffusion coefficient diffusion kurtosis imaging subtraction restricted diffusion bio-phantom cell
発行日 2019-07-25
出版物タイトル Molecular Medicine Reports
20巻
3号
出版者 Spandidos Publications
開始ページ 2963
終了ページ 2969
ISSN 1791-2997
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン publisher
PubMed ID 31524240
DOI 10.3892/mmr.2019.10523
Web of Science KeyUT 000482435500103
関連URL isVersionOf https://doi.org/10.3892/mmr.2019.10523
JaLCDOI 10.18926/11730
タイトル(別表記) Measurement of intracellular pH by flow cytometry using pH sensitive fluorescence dye, and influence of hyperthermia and amiloride derivatives on the intracellular pH
フルテキストURL 006_001_005.pdf
著者 浅海 淳一| 川崎 祥二| 黒田 昌宏| 竹田 芳弘| 平木 祥夫|
抄録 エールリッヒ腹水癌細胞とそのアドリアマイシン耐性細胞において蛍光pH指示薬2'、7'-bis-(2-carboxyethyl) carboxyfluorescein] (BCECF) の蛍光量をフローサイトメトリーで測定することによって細胞内pHの検量曲線を作成することができた。このことより、これらの細胞においてBCECFの蛍光量で細胞内pHの変化を簡易に比較できることを示唆した。さらに、温熱、Na(+)/H(+) exchanger の阻害例であるアミロライド[3,5-diamino-6-chloro-N-(diaminomethylene) pyrazinecarboxamide]、およびアミロライド誘導隊MH-12-43[N-amidino-3-amino-6-chloro-5-(N-ethyliso-propylamino) pyrazinecarboxyamide] の細胞内pHへの影響をエールリッヒ腹水癌細胞で観察した。37℃では、0.5mMアミロライド、0.05mM MH-12-43により細胞内pHは減少し、42℃処理によりさらに減少した。42℃において、0.05mM MH-12-43による細胞内pHの減少は、0.5mMアミロライドによる減少より大きかった。
抄録(別表記) We examined relationship between intensity of intracellular fluorescence of [2', 7'-bis-(2'-carboxyethyl) carboxyfluorescein] (BCECF) and intracellular pH in Ehrlich ascites tumor cells and their adriamycin-resistant strain, and found a good correlation between them at both strains. This suggests that changes in the intracellular pH on these strains may be obtained through measurement of intracellular fluorescence of BCECF by flow cytometry. Further, we examined influence of hyperthermia, 3, 5-diamino-6-chloro-N-(diaminomethylene)pyrazinecarboxamide (amiloride), an inhibitor of Na(+)/H(+) exchanger, and its derivative; N-amidino-3-amino-6-chloro-5-(N-ethylisopropylamino) pyrazinecarboxyamide (MH-12-43) on the intracellular pH in Ehrlich ascites tumor cells. The treatment of 0.5mM amiloride or 0.05mM MH-12-43 reduced intracellular pH at 37℃, while the more reduction was observed by the treatment at 42℃. The reduction of intracellular pH by 0.05mM MH-12-43 was more substantial than that of 0.5mM amiloride at 42℃.
キーワード BCECF 細胞内pH (Intracellular pH) フローサイトメトリー (Flow Cytometry) アミロライド (Amiloride) MH-12-43
出版物タイトル 岡山大学医療技術短期大学部紀要
発行日 1996-02-29
6巻
開始ページ 1
終了ページ 5
ISSN 0917-4494
言語 Japanese
論文のバージョン publisher
NAID 120002313855
JaLCDOI 10.18926/11843
タイトル(別表記) Relationship between intracellular uptake of adriamycin and membrane potential in ADR resistant Ehrlich ascites tumor cells
フルテキストURL 003_081_085.pdf
著者 浅海 淳一| 川崎 祥二| 西川 光治| 高献 書| 黒田 昌宏| 平木 祥夫|
抄録 We observed adiamycin (ADR) uptake and cellular transmembrane potential [amount of intracellular fluorescence of 3,3'- (Di-n-hexyl)- 2,2'- oxacarbocyanine iodide (NK-2280)] in ADR-resistant cells established from Ehrlich ascites tumor cells (EATC) and wild type EATC. In ADR-resistant cells, ADR uptake and the cellular transmembrane potential decreased as the degree of resistance increased. 4,4'- diisothiocyanatostilbene- 2,2'- disulfonic acid (DIDS) induced markedly decreases of ADR uptake and the cellular transmembrane potential. A good correlation was observed between ADR uptake and transmembrane potential in cultured cells.
キーワード Adriamycin Cell Membrane Potential Flow Cytometry ADR-Resistant Cells DIDS
出版物タイトル 岡山大学医療技術短期大学部紀要
発行日 1993-01-31
3巻
開始ページ 81
終了ページ 85
ISSN 0917-4494
言語 Japanese
論文のバージョン publisher
NAID 120002313508
JaLCDOI 10.18926/15198
タイトル(別表記) Measurement of sampling time in MRI and reconstruction of MR image
フルテキストURL 014_2_121_128.pdf
著者 加藤 博和| 花元 克巳| 川崎 祥二| 澁谷 光一| 黒田 昌宏| 小山 矩|
抄録 MRIにおいてk-spaceを構築するためにはエコー信号をサンプリング時間に従って採取する必要がある.このサンプリング時間を測定するために,FOV(field of view)の左半分にファントムを置くことにより階段関数となるスピン分布を生成した。得られたエコー信号を,階段関数をフ-リェ変換したときに得られるフ-リェ係数と比較することによりサンプリング時間を求めた。得られたサンプリング時間を用いて,MRI MULTI-TEST PHANTOMをMRI装置でスキャンすることにより得られたエコー信号をサンプリングしk-spaceを構築した。このk-spaceをフ-リェ逆変換することによりMRI MULTI-TEST PHANTOMの画像を再構成することができた。
抄録(別表記) Regarding the construction of k-space in MRI, it is necessary to extract the required data from the echo signals with the rate of sampling time. In order to measure the sampling time, a rectangular phantom was placed on the left side of the FOV in the body coil of an MRI equipment, and got analog signals in the sequence of spin echo. The sampling time was determined by comparing the measured signals with the Fourier coefficients obtained by transforming the step function which was similar to the profile of the FOV. The MRI MULTI-TEST PHANTOM was scanned, and the k-space was constructed with the extracted data from the echo signals with the rate of the determined sampling time. The MRI images of the MULTI-TEST PHANTOM were reconstructed by inverse-transforming the obtained k-space.
キーワード MRI フーリェ変換 (Fourier transform) 画像再構成 (reconstruction) サンプリング時間 (sampling time)
出版物タイトル 岡山大学医学部保健学科紀要
発行日 2004-03-31
14巻
2号
開始ページ 121
終了ページ 128
ISSN 1345-0948
言語 Japanese
論文のバージョン publisher
NAID 120002307209
JaLCDOI 10.18926/15215
タイトル(別表記) A Study of Reconstruction of Magnetic Resonance Image
フルテキストURL 013_2_077_082.pdf
著者 加藤 博和| 花元 克巳| 黒田 昌宏| 澁谷 光一| 川崎 祥二|
抄録 磁気共鳴は医学におけるイメージングにおいて重要なモダリティーであり,高度な数学的手法が画像の再構成に取り入れられている。画像再構成の数学的手法および画像再構成にいたるまでの数学的な過程を理解するこは,MRIを学ぶものにとって非常に重要であるが,成書において明確な説明はなされていない。ここでは,MR画像の再構成法を,複素関数と複素フ-リェ係数を用いて導いたので報告する。
抄録(別表記) Magnetic resonance is an important modality for medical imaging, and advanced mathematical techniques are introduced into the reconstruction of images. Clearly understandable explanations of the reconstruction of the MR image and of the mathematical derivation process have not been presented in the texts. Reconstruction of MR image is presented using the complex function and its relevant Fourier coefficients.
キーワード MRI フーリェ変換 (Fourier transform) 画像再構成 (Reconstruction) 周波数エンコード (Frequency encode) 位相画像 (Phase image)
出版物タイトル 岡山大学医学部保健学科紀要
発行日 2003-03-20
13巻
2号
開始ページ 77
終了ページ 82
ISSN 1345-0948
言語 Japanese
論文のバージョン publisher
NAID 120002307578
JaLCDOI 10.18926/15229
タイトル(別表記) 加温後のtsAF8細胞の細胞周期
フルテキストURL 012_2_101_106.pdf
著者 澁谷 光一| 川崎 祥二| 黒田 昌宏| 浅海 淳一| 加藤 博和| 平木 祥夫|
抄録 Thermotolerance in tsAF8 cells develops during incubation at 34℃ after heating at 45℃, while it is suppressed by the following incubation at a non-permissive temperature of 39.7℃ after the same heating. The incubation temperature after heating may affect the cell cycle and consequently thermotolerance. In the present study, a relationship between the thermotolerance and the cell cycle of tsAF8 was investigated. The cell cycle fractions and DNA synthesis were measured by flow cytometry using double staining with propidium iodide and bromodeoxyuridine. When the tsAF8 cells were heated at 45℃ for 20 min, and thereafter incubated at 34℃, bromodeoxyuridine uptake in the S phase cells (DNA synthesis) was recovered to 65.1% 6 h after the heating, and the cells showed gradual accumulation in the G(2)/M phase. When the cells were incubated at 39.7℃ after heating at 45℃ for 20 min, then showed inhibition of thermotolerance development, the DNA synthesis was recovered to 15.1% temporarily 6 h after the heating, but it became 0% after 12 h, and the cells did not remarkably accumulate in any phases of the cell cycle. This inhibition of DNA synthesis at 39.7℃ was considered to be the result of cell survival decreasing by a step-down heating. However, the relationship between the thermotolerance and the cell cycle was not found out in tsAF8 cells, because the cells did not accumulate in any phases of the cell cycle under the inhibitory condition of thermotolerance.
抄録(別表記) tsAF8細胞は45℃の加温後34℃で培養すると温熱耐性が速やかに発現するが,加温後,制限温度である39.7℃で培養すると温熱耐性の発現が抑制される。加温後の培養温度が細胞周期に影響し,その結果として温熱耐性発現に影響を与えている可能性があることから,今回,Propidium Iodide(PI)とbromodeoxyuridine(BrdU)でtsAF8細胞を二重染色し,フローサイトメトリーによって温熱耐性と細胞周期の関係の有無について調べた。tsAF8細胞を45℃20分の加温後34℃で培養すると,6時間後にはG(1)期の細胞が減少し,12時間後にはG(2)/M期への蓄積が見られた。しかし,加温後39.7℃で培養した場合には細胞周期の進行がほとんど見られなかった。BrdU の取込みは,加温せずに39.7℃で培養した場合には活発に行われ,また,45℃20分加温後34℃で培養した場合には,6時間後にはBrdUの取り込みは65.1%まで回復した。しかし,温熱耐性発現の抑制が観察される45℃20分加温後39.7℃で培養した場合には,BrdUの取込み量は6時間後に一時的に15.1%に回復するが,12時間後には取込み量はゼロとなった。BrdUの取り込みが阻害されたのはstep-down heatingの現象による細胞生存率の減少が原因だと考えられたが,温熱耐性発現の抑制が観察される条件下では細胞周期の特定の時期への集積がなかったことから,温熱耐性と細胞周期との関係はtsAF8細胞においては見い出されなかった。
キーワード thermotolerance (温熱耐性) hyperthermia (ハイパーサーミア) tsAF8 cell cycle (細胞周期)
出版物タイトル 岡山大学医学部保健学科紀要
発行日 2002-03-20
12巻
2号
開始ページ 101
終了ページ 106
ISSN 1345-0948
言語 English
論文のバージョン publisher
NAID 120002307264
JaLCDOI 10.18926/15251
タイトル(別表記) Relative biological effectiveness (RBE) and potential leathal damage repair (PLDR) of heavy-ion beam
フルテキストURL 009_2_075_081.pdf
著者 川崎 祥二| 澁谷 光一| 浅海 淳一| 小松 めぐみ| 黒田 昌宏| 平木 祥夫| 古澤 佳也|
抄録 150KV X線,中性子線及び炭素(LET13, 20, 50, 90, 140, 150, 153, 200keV/μm)を照射したマウスNIH3T3細胞の生存率曲線のLD(10)から(60)Coγ線に対する生物学的効果比(RBE)を求めた。RBEは150KV X線では1.26,中性子線では2.44,炭素線(LET13, 20, 50, 90, 140, 150, 153, 200keV/μm)ではそれぞれ1.41, 1.47, 2.22, 2.61, 1.61, 2.05, 1.57であった。LETとRBEの関係では100keV/μm付近にピークを認めた。150KVX線のLETは13keV/μm,中性子線のLETは70keVμmに相当した。(60)Co γ線の潜在性致死損傷からの回復(PLDR)は大きかった。炭素線(13keV/μm)照射でもPLDRが観察されるがLETが大きくなるとPLDRは減少したが,LET90keV/μmの炭素線でもPLDRが認められた。照射時の細胞状態の検討では増殖期の細胞の感受性は定常期細胞に比し僅かに高かった。
抄録(別表記) Relative biological effectiveness (RBE) and repair of potential lethal damage (PLDR) of NIH3T3 cells against heavy-ion radiation were studied. RBE of 150 KV X-rays and neutron estimated from LD(10) dose of dose response survival curves compared to (60)Co γ-ray were 1.26 and 2.44, respectively. RBE of 13, 20, 50, 90, 140, 150, 153, 200 keV/μm of LET of carbon beam were 1.41, 1.47, 2.22, 2.61, 2.61, 1.61, 2.05 and 1.57, respectively. Potential lethal damage repair (PLDR) after exposure to carbon beam was observed. The magnitude of PLDR of (60)Co γ-ray was the biggest. As for the carbon beam of LET of 13 keV/μm as well, PLDR were observed. PLDR decreased when LET of carbon beam grew big.
キーワード PLDR RBE Heavy-lon Radiation NIH3T3 Cells
出版物タイトル 岡山大学医療技術短期大学部紀要
発行日 1999-02-26
9巻
2号
開始ページ 75
終了ページ 81
ISSN 0917-4494
言語 Japanese
論文のバージョン publisher
NAID 120002307590
JaLCDOI 10.18926/AMO/31087
フルテキストURL fulltext.pdf
著者 Tanaka, Akio| Kuroda, Masahiro| Inamura, Keiji| Kawasaki, Shouji| Hiraki, Yoshio|
抄録 <p>External capacitive heating is the usual method of electromagnetic wave heating, in which the tumor is caught and heated between two opposite applicators. Using a phantom, the authors developed and evaluated the performance of a new capacitive heating applicator designed for simultaneous radiohyperthermotherapy (SRH) in which the electron beam irradiation is provided from above an external capacitive heating applicator for the treatment of superficial and shallow-seated tumors. The trial applicator was constructed to fulfill the following conditions: 1. use of an electrode plate which does not affect the electron beam depth dose, 2. a uniform thickness to maintain flatness of the electron beam, and 3. a cooling function to prevent damage to normal skin tissue and enhance the therapeutic gain factor. This applicator was comprised of a 0.1-mm-thick copper electrode and a 5-mm-thick cooling chamber. The depth of the 80% dose of the new applicator was 21 mm with a 9-MeV electron beam and 36mm with a 15-MeV electron beam, which was comparable to the effect of a conventional irradiation bolus. The temperature distribution produced by the trial applicator was symmetrical on both sides from the center of the applicator. The 50% specific absorption rate region was 6.4 cm wide at a depth of 1 cm from the phantom surface and 2.8 cm wide at a depth of 3 cm. There have been no previous reports on the development of an external capacitive heating applicator designed for the SRH of superficial and shallow-seated tumors; this is the first such report.(ABSTRACT TRUNCATED AT 250 WORDS)</p>
キーワード simultaneous radiohyperthermotherapy applicator capacitive heating superficial and shallowseated tumor
Amo Type Article
発行日 1994-08
出版物タイトル Acta Medica Okayama
48巻
4号
出版者 Okayama University Medical School
開始ページ 211
終了ページ 216
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7817776
Web of Science KeyUT A1994PE51400006
JaLCDOI 10.18926/AMO/31630
フルテキストURL fulltext.pdf
著者 Yamamoto, Michinori| Joja, Ikuo| Takemoto, Mitsuhiro| Kuroda, Masahiro| Hiraki, Yoshio|
抄録 We analyzed the influence of various parameters on the results of radiotherapy for T1 glottic cancer by assessing the outcomes of 60 patients with this cancer who received definitive radiotherapy between 1985 and 1994. Seven patients were treated with a cobalt-60 unit, and the other 53 with a linear accelerator (26 patients at 3-MV, 10 at 6-MV, and 17 at 10-MV). Of the 17 patients treated at 10-MV, 4 also received part of their treatment with a cobalt-60 unit. The total radiation dose ranged from 56 Gy to 70 Gy (mean, 61 Gy). The total radiation dose of 51 patients (85%) was 60 Gy. The factors found to influence local control were the strength of the radiation beam energy and whether or not there was gross tumor invasion of the anterior commissure. The local control rate was 71% in the patients treated with a 10-MV linear accelerator, 56% in those treated with a 6-MV linear accelerator and, 97% in those treated with a cobalt-60 unit or a 3-MV linear accelerator (P = 0.0173). The local control rate was 43% in the patients with gross anterior commissure invasion and 88% in those without (P = 0.0075). We conclude that low energy photon beams are more suitable for the treatment of early glottic cancers, especially if the lesion grossly invades the anterior commissure.
キーワード T1 glottic cancers radiotherapy radiation beam energy
Amo Type Article
発行日 1999-04
出版物タイトル Acta Medica Okayama
53巻
2号
出版者 Okayama University Medical School
開始ページ 91
終了ページ 94
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 Copyright© 1999 Okayama University Medical School
論文のバージョン publisher
査読 有り
Web of Science KeyUT 000080058700005
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/3496
JaLCDOI 10.18926/AMO/31990
フルテキストURL fulltext.pdf
著者 Togami, Izumi| Sasai, Nobuya| Tsunoda, Masatoshi| Sei, Tetsuro| Yabuki, Takayuki| Kitagawa, Takahiro| Mitani, Masahiko| Akaki, Shiro| Kuroda, Masahiro| Hiraki, Yoshio|
抄録 <p>A preliminary study was conducted to evaluate the superior and inferior glenoid labra with abductive movement using an open-type MR unit in asymptomatic healthy volunteers. Both fast low angle shot (FLASH) and turbo spin echo (TSE) images were obtained to evaluate the shapes of both the superior and inferior labra, as well as to assess changes in signal at these sites. As the abduction angle was increased, the shape of the superior labrum changed from round or triangular to crescentic and a higher signal was frequently seen. At an abduction angle of 150 degrees, an increase in signal was seen in one-half of the superior labra; this increase was noted more frequently in volunteers over 40 years of age. In some of the superior labra, the increase in signal seen at 150 degrees abduction disappeared on subsequent images obtained at 0 degrees abduction. Hence, the increase in signal was considered to be a reversible change. The shape of the inferior labrum tended to change from crescentic to triangular or round. An increase in signal in the inferior labrum was unrelated to the abduction angle. Abductive kinematic studies using an open-type MR unit provides information about the morphology of the superior and inferior labra, as well as information about signal changes occurring at these sites.</p>
キーワード shoulder kinematic magnetic resonance imaging(MRI) glenoid labrum open-type MRI
Amo Type Article
発行日 2001-08
出版物タイトル Acta Medica Okayama
55巻
4号
出版者 Okayama University Medical School
開始ページ 237
終了ページ 243
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 11512566
Web of Science KeyUT 000170367200006
JaLCDOI 10.18926/AMO/32638
フルテキストURL fulltext.pdf
著者 Kuroda, Masahiro| Inamura, Keiji| Tahara, Seiji| Mimura, Seiichi| Mikami, Yasutaka| Kawasaki, Shoji| Hiraki, Yoshio|
抄録 <p>Simultaneous radiohyperthermotherapy (SRH) is a combined hyperthermia-radiation therapy in which irradiation is given during heating. Mutual interference between the high energy radiotherapy system (Toshiba LMR-15A) and the 13.56 MHz capacitive heating system (Omron HEH-500C) was tested with phantom materials prior to a clinical trial with SRH. The energy and flatness of irradiation were not affected by the heating system within the range of clinical use. The high energy radiotherapy system did not affect the increase or distribution of temperature during simultaneous treatment. The results of this phantom study indicated that these apparatuses would not produce clinically significant mutual interference during SRH. A clinical trial was performed on a 57-year-old woman with postoperative recurrence of rectal cancer. This is the first reported clinical case treated with true SRH in which external irradiation was administered during mid capacitive heating. Twelve SRH treatments were performed on the recurrent lesion at a frequency of twice a week for six weeks using the apparatuses described above. There was a significant reduction in pain after treatment. The tumor marker carcinoembryonic antigen (CEA) level decreased after treatment. On CT images taken after treatment, the tumor site became a low density area which indicated necrosis. There were no side effects. These results suggest that further clinical study of SRH should be performed to clarify its advantages.</p>
キーワード hyperthermia capacitive heating radiotherapy phantom study simultaneous radio-hyperthermotherapy
Amo Type Article
発行日 1992-12
出版物タイトル Acta Medica Okayama
46巻
6号
出版者 Okayama University Medical School
開始ページ 417
終了ページ 426
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1485536
Web of Science KeyUT A1992KE49600003
JaLCDOI 10.18926/AMO/32672
フルテキストURL fulltext.pdf
著者 Kuroda, Masahiro| Kawasaki, Shoji| Hiraki, Yoshio|
抄録 <p>The effects of cepharanthin (Ce), glycyrrhizin (G), verapamil (V), and G plus V on induced thermotolerance in NIH3T3 cells were studied. Cells were heated with or without the drug at 45 degrees C for 20 min (the first heating), incubated at 37 degrees C for 12h (the incubation period), and heated again at 45 degrees C for 0-210 min (the second heating). G and V were added throughout the experiment, while Ce was added throughout the experiment or during only the first or second heating, or the incubation period. The cells were harvested after the second heating to evaluate cell survival. In control experiments without any drug, thermotolerance developed and reached the highest peak in the cells incubated for 12h at 37 degrees C. However, thermotolerance in the control cells was suppressed by incubating them at 0 degree C, but developed by subsequent incubation at 37 degrees C. This suggests that the acquisition of thermotolerance by the cells required metabolic processes during the incubation at 37 degrees C. When each drug was present throughout the experiment, only Ce or the combined use of G and V was effective in reducing thermotolerance. Thermotolerance was also suppressed in the presence of Ce during the second heating. These results indicate that Ce reduces thermotolerance by enhancing thermosensitivity rather than by inhibiting the development of thermotolerance.</p>
キーワード thermotolerance hyperthermia cepharanthin glycyrrhizin verapamil
Amo Type Article
発行日 1992-06
出版物タイトル Acta Medica Okayama
46巻
3号
出版者 Okayama University Medical School
開始ページ 147
終了ページ 155
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1502918
Web of Science KeyUT A1992JB50400001
JaLCDOI 10.18926/AMO/48559
フルテキストURL 66_3_203.pdf
著者 Ohno, Seiichiro| Harimoto, Takashi| Hirosue, Miyuki| Miyai, Masahiro| Hattori, Kengo| Kuroda, Masahiro| Kanazawa, Susumu| Inamura, Keiji| Kato, Hirokazu|
抄録 Magnetic resonance imaging (MRI) visualization of metallic stent lumens is possible if the stent structure counteracts eddy currents in the lumen induced by the radio frequency magnetic field, B1. To examine the effectiveness of various stent designs in counteracting eddy currents, we anchored eight copper stent models and 2 commercially available nickel-titanium alloy (Nitinol) stents in a gel phantom, perpendicular or parallel to the direction of B1. A mesh stent lumen showed hypointensity irrespective of its alignment relative to B1. A solenoid stent lumen showed hypointensity with the stent axis parallel to B1, but it had the same signal intensity as outside the lumen when perpendicular to B1. A Moebius stent lumen showed no signal reduction, irrespective of alignment relative to B1. Lumens of the commercially available stents showed hypointensity regardless of alignment relative to B1. Computer simulation revealed that the signal intensities of the stents corresponded to magnetic flux densities of B1 in the stents, which are modified by the structure of the stent. While in vivo MRI viewing of a Moebius stent lumen is likely possible regardless of axis alignment, inherent structural weakness may be problematic. As a more practical choice, the solenoid stent is easier to manufacture and generates no hypointensive signal when the axis is parallel to B0.
キーワード MRI visualization of stent lumen solenoid pattern Moebius pattern direction of B1
Amo Type Original Article
発行日 2012-06
出版物タイトル Acta Medica Okayama
66巻
3号
出版者 Okayama University Medical School
開始ページ 203
終了ページ 211
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 22729100
Web of Science KeyUT 000305669700003
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/48542
JaLCDOI 10.18926/AMO/48964
フルテキストURL 66_5_399.pdf
著者 Jing, Shao-Wu| Wang, Ya-Di| Kuroda, Masahiro| Su, Jing-Wei| Sun, Guo-Gui| Liu, Qing| Cheng, Yun-Jie| Yang, Cong-Rong|
抄録 Hypoxia-inducible factor-1α (HIF-1α) has been found to enhance tumor invasion and metastasis, but no study has reported its action in esophageal carcinoma. The goal of this study was to explore the probable mechanism of HIF-1α in the invasion and metastasis of esophageal carcinoma Eca109 cells in vitro and in vivo. mRNA and protein expression of HIF-1α, E-cadherin and matrix metalloproteinase-2 (MMP-2) under hypoxia were detected by RT-PCR and Western blotting. The effects of silencing HIF-1α on E-cadherin, MMP-2 mRNA and protein expression under hypoxia or normoxia were detected by RT-PCR and Western blotting, respectively. The invasive ability of Eca109 cells was tested using a transwell chambers. We established an Eca109-implanted tumor model and observed tumor growth and lymph node metastasis. The expression of HIF-1α, E-cadherin and MMP-2 in xenograft tumors was detected by Western blotting. After exposure to hypoxia, HIF-1α protein was up-regulated, both mRNA and protein levels of E-cadherin were down-regulated and MMP-2 was up-regulated, while HIF-1α mRNA showed no significant change. SiRNA could block HIF-1α effectively, increase E-cadherin expression and inhibit MMP-2 expression. The number of invading cells decreased after HIF-1α was silenced. Meanwhile, the tumor volume was much smaller, and the metastatic rate of lymph nodes and the positive rate were lower in vivo. Our observations suggest that HIF-1α inhibition might be an effective strategy to weaken invasion and metastasis in the esophageal carcinoma Eca109 cell line.
キーワード hypoxia-inducible factor-1α esophageal carcinoma invasion and metastasis
Amo Type Original Article
発行日 2012-10
出版物タイトル Acta Medica Okayama
66巻
5号
出版者 Okayama University Medical School
開始ページ 399
終了ページ 407
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 23093058
Web of Science KeyUT 000310253900004
著者 高 献書| 中川 富夫| 山本 道法| 黒田 昌宏| 栄 勝美| 清水 光春| 新屋 晴孝| 竹田 芳弘| 上者 郁夫| 平木 祥夫| 川崎 祥二|
発行日 1996-06-29
出版物タイトル 岡山医学会雑誌
108巻
3-6号
資料タイプ 学術雑誌論文
著者 黒田 昌宏| 浅海 淳一| 西川 光治| 田中 聖了| 高 献書| 山本 道法| 巻幡 栄一| 平木 祥夫| 川崎 祥二|
発行日 1993-02
出版物タイトル 岡山医学会雑誌
105巻
1-2号
資料タイプ 学術雑誌論文
著者 黒田 昌宏| 平木 祥夫| 川崎 祥二|
発行日 1991-08
出版物タイトル 岡山医学会雑誌
103巻
7-8号
資料タイプ 学術雑誌論文