JaLCDOI 10.18926/15251
タイトル(別表記) Relative biological effectiveness (RBE) and potential leathal damage repair (PLDR) of heavy-ion beam
フルテキストURL 009_2_075_081.pdf
著者 川崎 祥二| 澁谷 光一| 浅海 淳一| 小松 めぐみ| 黒田 昌宏| 平木 祥夫| 古澤 佳也|
抄録 150KV X線,中性子線及び炭素(LET13, 20, 50, 90, 140, 150, 153, 200keV/μm)を照射したマウスNIH3T3細胞の生存率曲線のLD(10)から(60)Coγ線に対する生物学的効果比(RBE)を求めた。RBEは150KV X線では1.26,中性子線では2.44,炭素線(LET13, 20, 50, 90, 140, 150, 153, 200keV/μm)ではそれぞれ1.41, 1.47, 2.22, 2.61, 1.61, 2.05, 1.57であった。LETとRBEの関係では100keV/μm付近にピークを認めた。150KVX線のLETは13keV/μm,中性子線のLETは70keVμmに相当した。(60)Co γ線の潜在性致死損傷からの回復(PLDR)は大きかった。炭素線(13keV/μm)照射でもPLDRが観察されるがLETが大きくなるとPLDRは減少したが,LET90keV/μmの炭素線でもPLDRが認められた。照射時の細胞状態の検討では増殖期の細胞の感受性は定常期細胞に比し僅かに高かった。
抄録(別表記) Relative biological effectiveness (RBE) and repair of potential lethal damage (PLDR) of NIH3T3 cells against heavy-ion radiation were studied. RBE of 150 KV X-rays and neutron estimated from LD(10) dose of dose response survival curves compared to (60)Co γ-ray were 1.26 and 2.44, respectively. RBE of 13, 20, 50, 90, 140, 150, 153, 200 keV/μm of LET of carbon beam were 1.41, 1.47, 2.22, 2.61, 2.61, 1.61, 2.05 and 1.57, respectively. Potential lethal damage repair (PLDR) after exposure to carbon beam was observed. The magnitude of PLDR of (60)Co γ-ray was the biggest. As for the carbon beam of LET of 13 keV/μm as well, PLDR were observed. PLDR decreased when LET of carbon beam grew big.
キーワード PLDR RBE Heavy-lon Radiation NIH3T3 Cells
出版物タイトル 岡山大学医療技術短期大学部紀要
発行日 1999-02-26
9巻
2号
開始ページ 75
終了ページ 81
ISSN 0917-4494
言語 Japanese
論文のバージョン publisher
NAID 120002307590
著者 佐藤 伸夫| 上田 裕之| 中村 哲也| 黒田 昌宏| 神崎 典子| 水田 昭文| 村上 公則| 上者 郁夫| 橋本 啓二| 平木 祥夫| 青野 要|
発行日 1987-08-30
出版物タイトル 岡山医学会雑誌
99巻
7-8号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/11730
タイトル(別表記) Measurement of intracellular pH by flow cytometry using pH sensitive fluorescence dye, and influence of hyperthermia and amiloride derivatives on the intracellular pH
フルテキストURL 006_001_005.pdf
著者 浅海 淳一| 川崎 祥二| 黒田 昌宏| 竹田 芳弘| 平木 祥夫|
抄録 エールリッヒ腹水癌細胞とそのアドリアマイシン耐性細胞において蛍光pH指示薬2'、7'-bis-(2-carboxyethyl) carboxyfluorescein] (BCECF) の蛍光量をフローサイトメトリーで測定することによって細胞内pHの検量曲線を作成することができた。このことより、これらの細胞においてBCECFの蛍光量で細胞内pHの変化を簡易に比較できることを示唆した。さらに、温熱、Na(+)/H(+) exchanger の阻害例であるアミロライド[3,5-diamino-6-chloro-N-(diaminomethylene) pyrazinecarboxamide]、およびアミロライド誘導隊MH-12-43[N-amidino-3-amino-6-chloro-5-(N-ethyliso-propylamino) pyrazinecarboxyamide] の細胞内pHへの影響をエールリッヒ腹水癌細胞で観察した。37℃では、0.5mMアミロライド、0.05mM MH-12-43により細胞内pHは減少し、42℃処理によりさらに減少した。42℃において、0.05mM MH-12-43による細胞内pHの減少は、0.5mMアミロライドによる減少より大きかった。
抄録(別表記) We examined relationship between intensity of intracellular fluorescence of [2', 7'-bis-(2'-carboxyethyl) carboxyfluorescein] (BCECF) and intracellular pH in Ehrlich ascites tumor cells and their adriamycin-resistant strain, and found a good correlation between them at both strains. This suggests that changes in the intracellular pH on these strains may be obtained through measurement of intracellular fluorescence of BCECF by flow cytometry. Further, we examined influence of hyperthermia, 3, 5-diamino-6-chloro-N-(diaminomethylene)pyrazinecarboxamide (amiloride), an inhibitor of Na(+)/H(+) exchanger, and its derivative; N-amidino-3-amino-6-chloro-5-(N-ethylisopropylamino) pyrazinecarboxyamide (MH-12-43) on the intracellular pH in Ehrlich ascites tumor cells. The treatment of 0.5mM amiloride or 0.05mM MH-12-43 reduced intracellular pH at 37℃, while the more reduction was observed by the treatment at 42℃. The reduction of intracellular pH by 0.05mM MH-12-43 was more substantial than that of 0.5mM amiloride at 42℃.
キーワード BCECF 細胞内pH (Intracellular pH) フローサイトメトリー (Flow Cytometry) アミロライド (Amiloride) MH-12-43
出版物タイトル 岡山大学医療技術短期大学部紀要
発行日 1996-02-29
6巻
開始ページ 1
終了ページ 5
ISSN 0917-4494
言語 Japanese
論文のバージョン publisher
NAID 120002313855
JaLCDOI 10.18926/11843
タイトル(別表記) Relationship between intracellular uptake of adriamycin and membrane potential in ADR resistant Ehrlich ascites tumor cells
フルテキストURL 003_081_085.pdf
著者 浅海 淳一| 川崎 祥二| 西川 光治| 高献 書| 黒田 昌宏| 平木 祥夫|
抄録 We observed adiamycin (ADR) uptake and cellular transmembrane potential [amount of intracellular fluorescence of 3,3'- (Di-n-hexyl)- 2,2'- oxacarbocyanine iodide (NK-2280)] in ADR-resistant cells established from Ehrlich ascites tumor cells (EATC) and wild type EATC. In ADR-resistant cells, ADR uptake and the cellular transmembrane potential decreased as the degree of resistance increased. 4,4'- diisothiocyanatostilbene- 2,2'- disulfonic acid (DIDS) induced markedly decreases of ADR uptake and the cellular transmembrane potential. A good correlation was observed between ADR uptake and transmembrane potential in cultured cells.
キーワード Adriamycin Cell Membrane Potential Flow Cytometry ADR-Resistant Cells DIDS
出版物タイトル 岡山大学医療技術短期大学部紀要
発行日 1993-01-31
3巻
開始ページ 81
終了ページ 85
ISSN 0917-4494
言語 Japanese
論文のバージョン publisher
NAID 120002313508
著者 原田 聡介| 武本 充広| 吉尾 浩太郎| 児島 克英| 片山 敬久| 勝井 邦彰| 黒田 昌宏| 松尾 俊彦| 金澤 右|
発行日 2010-12-01
出版物タイトル 岡山医学会雑誌
122巻
3号
資料タイプ 学術雑誌論文
著者 黒田 昌宏| 浅海 淳一| 西川 光治| 田中 聖了| 高 献書| 山本 道法| 巻幡 栄一| 平木 祥夫| 川崎 祥二|
発行日 1993-02
出版物タイトル 岡山医学会雑誌
105巻
1-2号
資料タイプ 学術雑誌論文
著者 高 献書| 中川 富夫| 山本 道法| 黒田 昌宏| 栄 勝美| 清水 光春| 新屋 晴孝| 竹田 芳弘| 上者 郁夫| 平木 祥夫| 川崎 祥二|
発行日 1996-06-29
出版物タイトル 岡山医学会雑誌
108巻
3-6号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/48559
フルテキストURL 66_3_203.pdf
著者 Ohno, Seiichiro| Harimoto, Takashi| Hirosue, Miyuki| Miyai, Masahiro| Hattori, Kengo| Kuroda, Masahiro| Kanazawa, Susumu| Inamura, Keiji| Kato, Hirokazu|
抄録 Magnetic resonance imaging (MRI) visualization of metallic stent lumens is possible if the stent structure counteracts eddy currents in the lumen induced by the radio frequency magnetic field, B1. To examine the effectiveness of various stent designs in counteracting eddy currents, we anchored eight copper stent models and 2 commercially available nickel-titanium alloy (Nitinol) stents in a gel phantom, perpendicular or parallel to the direction of B1. A mesh stent lumen showed hypointensity irrespective of its alignment relative to B1. A solenoid stent lumen showed hypointensity with the stent axis parallel to B1, but it had the same signal intensity as outside the lumen when perpendicular to B1. A Moebius stent lumen showed no signal reduction, irrespective of alignment relative to B1. Lumens of the commercially available stents showed hypointensity regardless of alignment relative to B1. Computer simulation revealed that the signal intensities of the stents corresponded to magnetic flux densities of B1 in the stents, which are modified by the structure of the stent. While in vivo MRI viewing of a Moebius stent lumen is likely possible regardless of axis alignment, inherent structural weakness may be problematic. As a more practical choice, the solenoid stent is easier to manufacture and generates no hypointensive signal when the axis is parallel to B0.
キーワード MRI visualization of stent lumen solenoid pattern Moebius pattern direction of B1
Amo Type Original Article
発行日 2012-06
出版物タイトル Acta Medica Okayama
66巻
3号
出版者 Okayama University Medical School
開始ページ 203
終了ページ 211
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 22729100
Web of Science KeyUT 000305669700003
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/48542
JaLCDOI 10.18926/AMO/31630
フルテキストURL fulltext.pdf
著者 Yamamoto, Michinori| Joja, Ikuo| Takemoto, Mitsuhiro| Kuroda, Masahiro| Hiraki, Yoshio|
抄録 We analyzed the influence of various parameters on the results of radiotherapy for T1 glottic cancer by assessing the outcomes of 60 patients with this cancer who received definitive radiotherapy between 1985 and 1994. Seven patients were treated with a cobalt-60 unit, and the other 53 with a linear accelerator (26 patients at 3-MV, 10 at 6-MV, and 17 at 10-MV). Of the 17 patients treated at 10-MV, 4 also received part of their treatment with a cobalt-60 unit. The total radiation dose ranged from 56 Gy to 70 Gy (mean, 61 Gy). The total radiation dose of 51 patients (85%) was 60 Gy. The factors found to influence local control were the strength of the radiation beam energy and whether or not there was gross tumor invasion of the anterior commissure. The local control rate was 71% in the patients treated with a 10-MV linear accelerator, 56% in those treated with a 6-MV linear accelerator and, 97% in those treated with a cobalt-60 unit or a 3-MV linear accelerator (P = 0.0173). The local control rate was 43% in the patients with gross anterior commissure invasion and 88% in those without (P = 0.0075). We conclude that low energy photon beams are more suitable for the treatment of early glottic cancers, especially if the lesion grossly invades the anterior commissure.
キーワード T1 glottic cancers radiotherapy radiation beam energy
Amo Type Article
発行日 1999-04
出版物タイトル Acta Medica Okayama
53巻
2号
出版者 Okayama University Medical School
開始ページ 91
終了ページ 94
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 Copyright© 1999 Okayama University Medical School
論文のバージョン publisher
査読 有り
Web of Science KeyUT 000080058700005
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/3496
著者 Maki, Yu| Murakami, Jun| Asaumi, Jun-ichi| Tsujigiwa, Hidetsugu| Nagatsuka, Hitoshi| Kokeguchi, Susumu| Fukui, Kazuhiro| Kawai, Noriko| Yanagi, Yoshinobu| Kuroda, Masahiro| Tanaka, Noriaki| Matsubara, Nagahide| Kishi, Kanji|
発行日 2005-7
出版物タイトル Oral Oncology
41巻
10号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/32638
フルテキストURL fulltext.pdf
著者 Kuroda, Masahiro| Inamura, Keiji| Tahara, Seiji| Mimura, Seiichi| Mikami, Yasutaka| Kawasaki, Shoji| Hiraki, Yoshio|
抄録 <p>Simultaneous radiohyperthermotherapy (SRH) is a combined hyperthermia-radiation therapy in which irradiation is given during heating. Mutual interference between the high energy radiotherapy system (Toshiba LMR-15A) and the 13.56 MHz capacitive heating system (Omron HEH-500C) was tested with phantom materials prior to a clinical trial with SRH. The energy and flatness of irradiation were not affected by the heating system within the range of clinical use. The high energy radiotherapy system did not affect the increase or distribution of temperature during simultaneous treatment. The results of this phantom study indicated that these apparatuses would not produce clinically significant mutual interference during SRH. A clinical trial was performed on a 57-year-old woman with postoperative recurrence of rectal cancer. This is the first reported clinical case treated with true SRH in which external irradiation was administered during mid capacitive heating. Twelve SRH treatments were performed on the recurrent lesion at a frequency of twice a week for six weeks using the apparatuses described above. There was a significant reduction in pain after treatment. The tumor marker carcinoembryonic antigen (CEA) level decreased after treatment. On CT images taken after treatment, the tumor site became a low density area which indicated necrosis. There were no side effects. These results suggest that further clinical study of SRH should be performed to clarify its advantages.</p>
キーワード hyperthermia capacitive heating radiotherapy phantom study simultaneous radio-hyperthermotherapy
Amo Type Article
発行日 1992-12
出版物タイトル Acta Medica Okayama
46巻
6号
出版者 Okayama University Medical School
開始ページ 417
終了ページ 426
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1485536
Web of Science KeyUT A1992KE49600003
フルテキストURL fulltext.pdf
著者 Katsui, Kuniaki| Ogata, Takeshi| Watanabe, Kenta| Katayama, Norihisa| Kuroda, Masahiro| Kiura, Katsuyuki| Hiraki, Takao| Maeda, Yoshinobu| Toyooka, Shinichi| Kanazawa, Susumu|
キーワード cisplatin docetaxel dose-volume histogram non-small cell lung cancer PACIFIC trial radiation pneumonitis
発行日 2020-05-04
出版物タイトル Cancer Medicine
出版者 Wiley
ISSN 2045-7634
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 © 2020 The Authors.
論文のバージョン publisher
PubMed ID 32364685
DOI 10.1002/cam4.3093
Web of Science KeyUT 000530411600001
関連URL isVersionOf https://doi.org/10.1002/cam4.3093
JaLCDOI 10.18926/AMO/30776
フルテキストURL fulltext.pdf
著者 Makihata, Eiichi| Kuroda, Masahiro| Kawai, Akira| Ozaki, Toshifumi| Sugihara, Shinsuke| Inoue, Hajime| Joja, Ikuo| Asaumi, Junichi| Kawasaki, Shoji| Hiraki, Yoshio|
抄録 <p>We report the results of phase I/II studies of preoperative multidisciplinary treatment of 14 patients with soft tissue sarcoma using hyperthermia from November 1990 to April 1995. The preoperative treatment was conducted with thermo-radio-chemotherapy in 11 cases of stage III, and with thermo-radiotherapy as well as thermo-chemotherapy in three cases of stages I and II. Hyperthermia was carried out twice a week with totals ranging from 4 to 14 times (average: 8.4 times); each session lasted 60min. Radiotherapy was administered four or five times per week, and the dose was 1.8 2Gy/fraction, with a total of 30-40Gy in a four week period. Chemotherapy was mainly in the form of MAID regimen (2-mercaptoethanesulphonic acid (mesna), adriamycin, ifosfamide and dacarbazine). The tumors were surgically resected in all patients after completing the preoperative treatment. The efficacy rate, as expressed by the percentage of either tumors in which reduction rate was 50% or more, or tumors for which post-treatment contrast enhanced CT image revealed low density volumes occupying 50% or more of the total mass, was 71 % (ten of the 14 tumors). The mean tumor necrosis rate in the resected specimens was 78%. The tumor necrosis rate was significantly high (P &#60; 0.05) in patients whose Time &#8805; 42°C was of long duration. Postoperative complications were observed in six patients; among these, two patients developed wound infection that required surgical treatment as a complication of surgery performed in the early stage following the preoperative treatment. After a mean postoperative follow-up of 27 months, distant metastasis occurred in four patients resulting in three fatalities. The three-year cumulative survival rate was 64.3%. No local recurrence was observed in any patient during the follow-up, thus confirming our hypothesis that preoperative multidisciplinary treatment has an excellent local efficacy. We think that it would be valuable to conduct, at many facilities, phase III studies on the treatment of soft tissue sarcoma by a combination of surgery and preoperative multidisciplinary treatment using hyperthermia, paying close attention to the interval between these two modalities.</p>
キーワード soft tissue tumor hyperthermia radiotherapy chemotherapy
Amo Type Article
発行日 1997-04
出版物タイトル Acta Medica Okayama
51巻
2号
出版者 Okayama University Medical School
開始ページ 93
終了ページ 99
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9142346
Web of Science KeyUT A1997WX19600006
著者 黒田 昌宏| 平木 祥夫| 川崎 祥二|
発行日 1991-08
出版物タイトル 岡山医学会雑誌
103巻
7-8号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/15215
タイトル(別表記) A Study of Reconstruction of Magnetic Resonance Image
フルテキストURL 013_2_077_082.pdf
著者 加藤 博和| 花元 克巳| 黒田 昌宏| 澁谷 光一| 川崎 祥二|
抄録 磁気共鳴は医学におけるイメージングにおいて重要なモダリティーであり,高度な数学的手法が画像の再構成に取り入れられている。画像再構成の数学的手法および画像再構成にいたるまでの数学的な過程を理解するこは,MRIを学ぶものにとって非常に重要であるが,成書において明確な説明はなされていない。ここでは,MR画像の再構成法を,複素関数と複素フ-リェ係数を用いて導いたので報告する。
抄録(別表記) Magnetic resonance is an important modality for medical imaging, and advanced mathematical techniques are introduced into the reconstruction of images. Clearly understandable explanations of the reconstruction of the MR image and of the mathematical derivation process have not been presented in the texts. Reconstruction of MR image is presented using the complex function and its relevant Fourier coefficients.
キーワード MRI フーリェ変換 (Fourier transform) 画像再構成 (Reconstruction) 周波数エンコード (Frequency encode) 位相画像 (Phase image)
出版物タイトル 岡山大学医学部保健学科紀要
発行日 2003-03-20
13巻
2号
開始ページ 77
終了ページ 82
ISSN 1345-0948
言語 Japanese
論文のバージョン publisher
NAID 120002307578
JaLCDOI 10.18926/15198
タイトル(別表記) Measurement of sampling time in MRI and reconstruction of MR image
フルテキストURL 014_2_121_128.pdf
著者 加藤 博和| 花元 克巳| 川崎 祥二| 澁谷 光一| 黒田 昌宏| 小山 矩|
抄録 MRIにおいてk-spaceを構築するためにはエコー信号をサンプリング時間に従って採取する必要がある.このサンプリング時間を測定するために,FOV(field of view)の左半分にファントムを置くことにより階段関数となるスピン分布を生成した。得られたエコー信号を,階段関数をフ-リェ変換したときに得られるフ-リェ係数と比較することによりサンプリング時間を求めた。得られたサンプリング時間を用いて,MRI MULTI-TEST PHANTOMをMRI装置でスキャンすることにより得られたエコー信号をサンプリングしk-spaceを構築した。このk-spaceをフ-リェ逆変換することによりMRI MULTI-TEST PHANTOMの画像を再構成することができた。
抄録(別表記) Regarding the construction of k-space in MRI, it is necessary to extract the required data from the echo signals with the rate of sampling time. In order to measure the sampling time, a rectangular phantom was placed on the left side of the FOV in the body coil of an MRI equipment, and got analog signals in the sequence of spin echo. The sampling time was determined by comparing the measured signals with the Fourier coefficients obtained by transforming the step function which was similar to the profile of the FOV. The MRI MULTI-TEST PHANTOM was scanned, and the k-space was constructed with the extracted data from the echo signals with the rate of the determined sampling time. The MRI images of the MULTI-TEST PHANTOM were reconstructed by inverse-transforming the obtained k-space.
キーワード MRI フーリェ変換 (Fourier transform) 画像再構成 (reconstruction) サンプリング時間 (sampling time)
出版物タイトル 岡山大学医学部保健学科紀要
発行日 2004-03-31
14巻
2号
開始ページ 121
終了ページ 128
ISSN 1345-0948
言語 Japanese
論文のバージョン publisher
NAID 120002307209
JaLCDOI 10.18926/AMO/31990
フルテキストURL fulltext.pdf
著者 Togami, Izumi| Sasai, Nobuya| Tsunoda, Masatoshi| Sei, Tetsuro| Yabuki, Takayuki| Kitagawa, Takahiro| Mitani, Masahiko| Akaki, Shiro| Kuroda, Masahiro| Hiraki, Yoshio|
抄録 <p>A preliminary study was conducted to evaluate the superior and inferior glenoid labra with abductive movement using an open-type MR unit in asymptomatic healthy volunteers. Both fast low angle shot (FLASH) and turbo spin echo (TSE) images were obtained to evaluate the shapes of both the superior and inferior labra, as well as to assess changes in signal at these sites. As the abduction angle was increased, the shape of the superior labrum changed from round or triangular to crescentic and a higher signal was frequently seen. At an abduction angle of 150 degrees, an increase in signal was seen in one-half of the superior labra; this increase was noted more frequently in volunteers over 40 years of age. In some of the superior labra, the increase in signal seen at 150 degrees abduction disappeared on subsequent images obtained at 0 degrees abduction. Hence, the increase in signal was considered to be a reversible change. The shape of the inferior labrum tended to change from crescentic to triangular or round. An increase in signal in the inferior labrum was unrelated to the abduction angle. Abductive kinematic studies using an open-type MR unit provides information about the morphology of the superior and inferior labra, as well as information about signal changes occurring at these sites.</p>
キーワード shoulder kinematic magnetic resonance imaging(MRI) glenoid labrum open-type MRI
Amo Type Article
発行日 2001-08
出版物タイトル Acta Medica Okayama
55巻
4号
出版者 Okayama University Medical School
開始ページ 237
終了ページ 243
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 11512566
Web of Science KeyUT 000170367200006
JaLCDOI 10.18926/AMO/52009
フルテキストURL 67_6_359.pdf
著者 Katashima, Kazunori| Kuroda, Masahiro| Ashida, Masakazu| Sasaki, Takanori| Taguchi, Takehito| Matsuzaki, Hidenobu| Murakami, Jun| Yanagi, Yoshinobu| Hisatomi, Miki| Hara, Marina| Kato, Hirokazu| Ohmura, Yuichi| Kobayashi, Tomoki| Kanazawa, Susumu| Harada, Sosuke| Takemoto, Mitsuhiro| Ohno, Seiichiro| Mimura, Seiichi| Asaumi, Junichi|
抄録 It is well known that many tumor tissues show lower apparent diffusion coefficient (ADC) values, and that several factors are involved in the reduction of ADC values. The aim of this study was to clarify how much each factor contributes to decreases in ADC values. We investigate the roles of cell density, extracellular space, intracellular factors, apoptosis and necrosis in ADC values using bio-phantoms. The ADC values of bio-phantoms, in which Jurkat cells were encapsulated by gellan gum, were measured by a 1.5-Tesla magnetic resonance imaging device with constant diffusion time of 30sec. Heating at 42℃ was used to induce apoptosis while heating at 48℃ was used to induce necrosis. Cell death after heating was evaluated by flow cytometric analysis and electron microscopy. The ADC values of bio-phantoms including non-heated cells decreased linearly with increases in cell density, and showed a steep decline when the distance between cells became less than 3μm. The analysis of ADC values of cells after destruction of cellular structures by sonication suggested that approximately two-thirds of the ADC values of cells originate from their cellular structures. The ADC values of bio-phantoms including necrotic cells increased while those including apoptotic cells decreased. This study quantitatively clarified the role of the cellular factors and the extracellular space in determining the ADC values produced by tumor cells. The intermediate diffusion time of 30msec might be optimal to distinguish between apoptosis and necrosis.
キーワード ADC apoptosis necrosis hyperthermia cell density
Amo Type Original Article
発行日 2013-12
出版物タイトル Acta Medica Okayama
67巻
6号
出版者 Okayama University Medical School
開始ページ 359
終了ページ 367
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2013 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 24356720
Web of Science KeyUT 000328915700004
JaLCDOI 10.18926/AMO/48566
フルテキストURL 66_3_263.pdf
著者 Sasaki, Takanori| Kuroda, Masahiro| Katashima, Kazunori| Ashida, Masakazu| Matsuzaki, Hidenobu| Asaumi, Junichi| Murakami, Jun| Ohno, Seiichiro| Kato, Hirokazu| Kanazawa, Susumu|
抄録 The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkittセs lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in vitro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values.
キーワード apparent diffusion coefficient value cell density extracellular space bio-phantom
Amo Type Original Article
発行日 2012-06
出版物タイトル Acta Medica Okayama
66巻
3号
出版者 Okayama University Medical School
開始ページ 263
終了ページ 270
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 22729107
Web of Science KeyUT 000305669700010
JaLCDOI 10.18926/AMO/31557
フルテキストURL fulltext.pdf
著者 Kuroda, Masahiro| Hizuta, Akio| Iwagaki, Hiromi| Makihata, Eiichi| Asaumi, Junichi| Nishikawa, Koji| Gao, Xian Shu| Nakagawa, Tomio| Togami, Izumi| Takeda, Yoshihiro| Joja, Ikuo| Kawasaki, Shoji| Orita, Kunzo| Hiraki, Yoshio|
抄録 <p>Between November 1984 and August 1992 we used hyperthermotherapy in six cases of local recurrence of rectal cancer. Hyperthermotherapy was performed on the average 8.7 times (range: 3-18) for each patient for 60 min each. All patients underwent combined radiotherapy and received a mean radiation dose of 42.5 Gy (range: 9-60 Gy). Five patients underwent heating within 1 h after irradiation and one patient simultaneously with the irradiation. Four patients underwent combined chemotherapy and two patients immunotherapy. Before the treatment all patients had painful lesions, but pain decreased posttherapeutically in five patients. Performance status improved in two patients. High carcinoembryonic antigen levels prior to the therapy in four patients decreased in all cases after treatment. Posttherapeutical computed tomograms revealed only minor response or no changes. After the treatment, four patients died of exacerbations of recurrent tumors and one patient of distant metastases. The patient who underwent simultaneous radiohyperthermotherapy is presently alive, in August 1992, 38 months after initiation of the treatment. The 50% survival time after initiation of the treatment was 25 months (range: 10-38 months). Hyperthermotherapy combined with radiotherapy, chemotherapy and/or immunotherapy was useful for the alleviation of pain in patients who developed local recurrence after surgery, and improved survival after recurrences can be expected.</p>
キーワード rectal cancer local recurrence hyperthermia radiotherapy chemotherapy
Amo Type Article
発行日 1993-08
出版物タイトル Acta Medica Okayama
47巻
4号
出版者 Okayama University Medical School
開始ページ 249
終了ページ 254
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8213219
Web of Science KeyUT A1993LV73800005