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ID 46909
フルテキストURL
著者
Furumatsu, Takayuki Department of Orthopaedic Surgery, Okayama University Graduate School
Ozaki, Toshifumi Department of Orthopaedic Surgery, Okayama University Graduate School
Asahara, Hiroshi Department of Molecular and Experimental Medicine, The Scripps Research Institute
抄録
Transforming growth factor (TGF)-β has an essential role for the Sry-type high-mobility-group box (Sox)-regulated chondrogenesis. Chondrogenic differentiation is also controlled by chromatin-mediated transcription. We have previously reported that TGF-β-regulated Smad3 induces chondrogenesis through the activation of Sox9-dependent transcription. However, the cross-talk between TGF-β signal and Sox9 on chromatin-mediated transcription has not been elucidated. In the present study, we investigated the activity of Smad3, Sox9, and coactivator p300 using an in vitro chromatin assembly model. Luciferase reporter assays revealed that Smad3 stimulated the Sox9-mediated transcription in a TGF-β-dependent manner. Recombinant Sox9 associated with phosphorylated Smad3/4 and recognized the enhancer region of type II collagen gene. In vitro transcription and S1 nuclease assays showed that Smad3 and p300 cooperatively activated the Sox9-dependent transcription on chromatin template. The combination treatment of phosphorylated Smad3, Sox9, and p300 were necessary for the activation of chromatin-mediated transcription. These findings suggest that TGF-β signal Smad3 plays a key role for chromatin remodeling to induce chondrogenesis via its association with Sox9.
キーワード
Chondrogenesis
Chromatin
p300
Smad3
Sox9
発行日
2009-05
出版物タイトル
The International Journal of Biochemistry & Cell Biology
41巻
5号
出版者
Pergamon-Elsevier Science Ltd.
開始ページ
1198
終了ページ
1204
ISSN
1357-2725
NCID
AA11036038
資料タイプ
学術雑誌論文
言語
English
著作権者
© 2008 Elsevier Ltd. All rights reserved.
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