JaLCDOI 10.18926/AMO/31026
フルテキストURL fulltext.pdf
著者 Senoo, Yoshimasa| Katoh, Kazuo| Nakai, Yumi| Hashimoto, Yutaka| Bando, Ko| Teramoto, Shigeru|
抄録 <p>The stability of recombinant human superoxide dismutase (r-hSOD) in buffer solutions was studied in solutions at various pH and temperatures. Additionally, we studied the effects of incubation with proteases, serum and two types of hypothermic perfusates. R-hSOD was stable in the pH range of 6-11 and at temperatures up to 80 degrees C for 30 min. R-hSOD activity was not affected by incubation with trypsin, aminopeptidase M or serum for 2 h. R-hSOD activity determined at various temperatures (4-37 degrees C) did not vary remarkably. R-hSOD in hypothermic perfusates was stable at 4-37 degrees C for 24 h.</p>
キーワード recombinant human superoxide dismutase activity stability hypothermic perfusate
Amo Type Article
発行日 1988-06
出版物タイトル Acta Medica Okayama
42巻
3号
出版者 Okayama University Medical School
開始ページ 169
終了ページ 174
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3041738
Web of Sience KeyUT A1988P034000007
JaLCDOI 10.18926/AMO/30513
フルテキストURL fulltext.pdf
著者 Takagaki, Masami| Hisamochi, Kunikazu| Morimoto, Toru| Bando, Ko| Sano, Shunji| Shimizu, Nobuyoshi|
抄録 <p>A shortage of donor organs in clinical transplantation prompted us to study whether resuscitated dead hearts could be utilized for successful orthotopic heart transplantation. After 60 min of hypoxic cardiac arrest, one group of canine hearts was resuscitated (Res group, n = 6). The other group was harvested directly (Non-Res group, n = 6). In the Res group, cardiopulmonary bypass was utilized for resuscitation at 37 degrees C and the animals were then core-cooled to 15 degrees C. The hearts then were preserved in University of Wisconsin solution and orthotopically transplanted. Stable prostacyclin analogue (OP2507) and verapamil, a calcium antagonist, were added to the cardioplegia, and substrate-enriched warm blood cardioplegia and a hydroxy radical scavenger (EPC) were administered at the time of reperfusion of the transplanted heart. All animals in each group were successfully weaned from cardiopulmonary bypass with dopamine (5 micrograms/kg/min). Cardiac function without dopamine was better preserved in the Res group than the Non-Res group (Emax: 130.6 +/- 41.5% vs. 47.1 +/- 24.7%; mean +/- SD, as percent of postbrain death values, P &#60; 0.01 by unpaired t-test). Cadaver hearts 60 min after anoxic arrest can be successfully re-animated and orthotopically engrafted. In addition, the core-cooling technique is useful. We believe this study serves as the key step in the clinical application of dead hearts to successful cardiac transplantation.</p>
キーワード heart transplantation cadaver heart corecooling Emax
Amo Type Article
発行日 1996-02
出版物タイトル Acta Medica Okayama
50巻
1号
出版者 Okayama University Medical School
開始ページ 17
終了ページ 24
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8701777
Web of Sience KeyUT A1996TY06000003