JaLCDOI 10.18926/AMO/31563
フルテキストURL fulltext.pdf
著者 Nouso, Kazuhiro| Battula, Narayama| Thorgeirsson, Snorri S| Higashi, Toshihiro| Tsuji, Takao|
抄録 <p>We expressed mouse cytochrome P1-450 and P3-450 using recombinant vaccinia virus gene expression system in HeLa cells that were devoid of significant basal levels of P-450. HeLa cells were infected with the recombinant vaccinia virus containing either mouse cytochrome P1-450 or P3-450 cDNA, and the cell lysates were analyzed for the kinetics of P-450 enzyme activity and protein expression at the same time. 7-Ethoxycoumarin O-deethylase and ethoxyresorufin O-deethylase activities were measured as an expression of the cytochrome P-450 enzyme activities. Both cell lines began to express these enzyme activities as early as 12h after infection. The activities increased linearly up to the 24 h time point, and were kept for 36 h. Western immunoblot analysis showed that these cytochrome P-450 proteins were detected at 16 h and reached maximum quantity at 24 h after infection. These data showed a good correlation between cytochrome P-450 enzyme activity and protein concentration throughout the process of P-450 gene expression by vaccinia virus vector, suggesting a complete formation of cytochrome P-450 holoenzyme from the early stage of the protein expression.</p>
キーワード cytochrome P-450 vaccinia virus kinetics
Amo Type Article
発行日 1993-12
出版物タイトル Acta Medica Okayama
47巻
6号
出版者 Okayama University Medical School
開始ページ 377
終了ページ 382
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8128911
Web of Sience KeyUT A1993MP00700004