JaLCDOI 10.18926/AMO/30947
フルテキストURL fulltext.pdf
著者 Khan, Rasel| Takahashi, Eizo| Nakura, Hironori| Ansaruzzaman, Mohammad| Banik, Sukalyani| Ramamurthy, Thandavarayan| Okamoto, Keinosuke|
抄録 <p>Aeromonas are water-borne pathogens. They are halotolerant, which means that they can survive in environments whose salt content corresponds to that of seawater (3.0% NaCl). However, the presence of Aeromonas in seawater is extremely rare compared with that in river water. In this study, we tested the ability of Aeromonas sobria to produce toxins in river water and seawater. First, we cultured A. sobria on skim milk agar plates supplemented with either river water (SARW) or seawater (SASW). The bacteria grew on both plates. A clear zone around the bacteria was generated in SARW. However, such a zone was not observed in SASW, suggesting that proteases were not generated in SASW. Subsequently, we cultured A. sobria in a nutrient broth supplemented with either river water (NRW) or with seawater (NSW), and examined the protease activity of their culture supernatants. The protease activity of the culture supernatant from NSW was extremely low compared to that from NRW. The immunoblotting analysis showed that serine protease (ASP) was not produced by the culture in NSW. By contrast, aerolysin-like hemolysin was produced in all conditions examined in this study. This indicates that the salinity of water is deeply involved in the production of ASP by A. sobria.</p>
キーワード Aeromonas water toxin salinity
Amo Type Original Article
発行日 2008-12
出版物タイトル Acta Medica Okayama
出版者 Okayama University Medical School
開始ページ 363
終了ページ 371
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
Web of Science KeyUT 000262025000002