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ID 30801
JaLCDOI
フルテキストURL
著者
Chen, Hong Li Okayama University
Hayashi, Kazuhiko Okayama University
Koirala, Tirtha Raj Okayama University
Ino, Hideo Okayama University
Fuimoto, Koji Tsukuba Primate Center for Medical Science
Yoshikawa, Yasuhiro Tsukuba Primate Center for Medical Science
Choudhury, Chitta Ranjan Okayama University
Akagi, Tadaatsu Okayama University
抄録

Malignant lymphoma was induced in Japanese (JWY), New Zealand (NZY) and Dutch (DUY) white rabbits by oral spray of cell-free pellets of culture fluid (crude virus fraction) of Ts-B6 cells (cynomolgus monkey B-lymphoblastoid cells harboring Epstein Barr virus-related simian herpesvirus or Cyno-EBV). Nine of 11 inoculated rabbits developed malignant lymphomas within 42-160 days after oral inoculation (JWY, 2/3; NZY, 5/6; DUY, 2/2). In contrast, none of the control rabbits inoculated in the same fashion with B95-8 (EBV-producing marmoset cell line) cell-free pellets developed malignant lymphoma. Most rabbits showed increased anti-VCA IgG and anti-EA-DR IgG antibody titers after inoculation by oral spray of Ts-B6 cell-free pellets. EBV-encoded RNA-1 was revealed in the tumor cells by in situ hybridization. EBV DNA was detected in the rabbit peripheral blood leukocytes (PBL) by polymerase chain reaction; the earliest positive result was obtained only two days after oral inoculation. These data suggest that orally administered Cyno-EBV in Ts-B6 cells infects PBL and then induces malignant lymphoma in rabbits. The availability of this animal model promises to clarify the role of EBV in human lymphoma and provides a means for studying prophylactic and therapeutic regimens.

キーワード
Epstein-Barr virus
malignant lymphoma
oral inoculation
simian herpesvirus
animal model
Amo Type
Article
発行日
1997-06
出版物タイトル
Acta Medica Okayama
51巻
3号
出版者
Okayama University Medical School
開始ページ
141
終了ページ
147
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT