JaLCDOI 10.18926/AMO/32178
フルテキストURL fulltext.pdf
著者 Sacho, Masanori| Setsu, Koujun| Hayashi, Keiki|
抄録 <p>Hematoxylin and eosin (H-E) stained liver sections of 47 autopsy cases of hepatic malignancies were examined. There were 43 cases of hepatocellular carcinoma (subtypes of 30 trabecular, 7 solid, 5 pseudoglandular, and one scirrhous carcinoma), 3 of cholangiocellular carcinoma and one of mixed carcinoma. After immunohistochemical staining, benign hepatocytes reacted positively with anti-epithelial membrane antigen (EMA). Hepatocellular carcinoma cells reacted more weakly than benign hepatocytes. It was noted that the microtubular structure, which could not be demonstrated even by alcian blue or cationic ferric hydroxide colloid stabilized with cacodylate (Fe-CaC), was clearly detected with anti-EMA. The EMA-positive microtubular structures may indicate terminal cholangiolar differentiation. Based on EMA, seven more cases formerly classified as hepatocellular carcinoma by H-E were reclassified as mixed carcinoma, totaling eight (17.0%). The histologic classification of &#34;mixed carcinoma&#34; has been 1.5 to 2.0% of primary liver cancers in Japan, but we suggest there may be more cases of &#34;mixed carcinoma&#34; identified in the future. In conclusion, we emphasize that EMA staining is useful for more accurate classification of hepatic tumors.</p>
キーワード primary liver carcinoma immunohistochemistry histochemistory epithelial membrane antigen
Amo Type Article
発行日 1991-12
出版物タイトル Acta Medica Okayama
45巻
6号
出版者 Okayama University Medical School
開始ページ 423
終了ページ 432
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1723562
Web of Sience KeyUT A1991GX45300004
JaLCDOI 10.18926/AMO/30896
フルテキストURL fulltext.pdf
著者 Hayashi, Keiki| Hsueh, Chao-Liang| Kawasaki, Hideaki| Toyofuku, Hiroyuki| Miyashima, Takanao| Awai, Michiyasu|
抄録 <p>Renal tissues from 208 human necropsies were observed histologically for disseminated intravascular coagulation (DIC). The tissues were stained with hematoxylin-eosin, Mallory's phosphotungstic acid hematoxylin (PTAH) and cationic ferric hydroxide colloid stabilized with cacodylate (Fe-Cac), and tested by immunoenzyme histochemical (IEH) reaction for fibrin-related materials (FRMs). The use of the IEH method increased FRM recognition, and FRMs were detected in a total of 80 cases (38.5%). In 26 cases diagnosed clinically as DIC, FRMs were shown in 23 of the cases (88.5%). Thus, 57 patients with FRMs were clinically asymptomatic. In rats with DIC induced by endotoxin injection, glomerulus FRM was effluxed into the tubulus through the Bowman's capsule and was excreted into urine. The electric charge was reduced on the endothelial surface of the glomerular capillaries in both human and rat DIC. Under the scanning electron microscopy, the endothelial surface appeared coarse in the glomerular capillary and fibrin degradation was present. Our conclusions are: (a) PTAH is non-specific for FRMs, (b) IEH aids the pathohistological diagnosis of DIC, especially in asymptomatic forms including the compensated DIC state, (c) FRMs in tubuli suggest DIC, and (d) DIC is possibly initiated by a reduction in the capillary electric surface charge.</p>
キーワード disseminated intravascular coagulation immunoenzyme-histochemistry fibrinrelated materials
Amo Type Article
発行日 1989-02
出版物タイトル Acta Medica Okayama
43巻
1号
出版者 Okayama University Medical School
開始ページ 29
終了ページ 38
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2470234
Web of Sience KeyUT A1989T938500004
JaLCDOI 10.18926/AMO/30868
フルテキストURL fulltext.pdf
著者 Miyashima, Takanao| Hayashi, Keiki| Awai, Michiyasu|
抄録 <p>To clarify the initiation, development and recovery processes of disseminated intravascular coagulation (DIC), rat glomerular capillaries and fibrin thrombi were examined under transmission and scanning electron microscopes. DIC was induced in rats by a single intraperitoneal injection of endotoxin (Et., 7.5 mg/kg lipopolysaccharide:B, E. coli 026:B6). At 2 h after Et. injection, the endothelial surface of the glomerular capillary became irregular with projections like a sea anemone. At 4 h after Et. injection, agglomerated fibrin thrombi composed of fibrin fiber bundles with fine cross-striated fibriform structures were observed in the capillary lumen. The fibrin thrombi gradually changed into fine reticular systems suggesting a degradation process by 6 h after Et. injection, and formed a coarse granular agglomerate by 8 h after Et. injection. These fibrin thrombi disappeared within 12 h of Et. injection, but the endothelial surface remained edematous. At 24 h after Et. injection, the microstructure of the glomerular capillaries returned normal. Based on these observations, we concluded that DIC was primarily initiated by injury to the capillary endothelium, and that changes on the endothelial surface contributed to the development of DIC.</p>
キーワード disseminated intravascular coagulation renal tissue electron microscope rat
Amo Type Article
発行日 1989-04
出版物タイトル Acta Medica Okayama
43巻
2号
出版者 Okayama University Medical School
開始ページ 115
終了ページ 126
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2728905
Web of Sience KeyUT A1989U578500006
JaLCDOI 10.18926/AMO/30867
フルテキストURL fulltext.pdf
著者 Xian, Mei-Sheng| Hayashi, Keiki| Lu, Jian-Ping| Awai, Michiyasu|
抄録 <p>Three types of traditional Chinese herb medicine were used to treat 98 patients with advanced esophageal squamous cell carcinoma prior to surgical treatment. Forty-two patients with the same diagnosis were treated with these herbs plus cyclophosphamide (endoxan). One hundred similar patients received surgical treatment without herbs or endoxan treatment as controls. Histologic examinations of surgical specimens were made on all of these patients. Stromal lymphoid-cell infiltration and cancer tissue degeneration were more prominent in Menispernum dehuricum DC- or Chelidonium majus L-treated patients, and were less clear in patients treated with herbs plus endoxan and the controls. The antitumor action of herbs is thought to be brought about by the activation of an immunological rejection mechanism. Herbs plus endoxan may result in the masking of the immunological response of hosts without obviously damaging cancer tissues.</p>
キーワード esophageal cancer Chinese herbs histopathology immunological response
Amo Type Article
発行日 1989-12
出版物タイトル Acta Medica Okayama
43巻
6号
出版者 Okayama University Medical School
開始ページ 345
終了ページ 351
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2624142
Web of Sience KeyUT A1989CG27400005