| JaLCDOI | 10.18926/AMO/31624 |
|---|---|
| フルテキストURL | fulltext.pdf |
| 著者 | Mizobuchi, Noriko| Takahashi, Isao| Yorimitsu, Seiichi| Horimi, Tadashi| Hamada, Kyoko| Matsuoka, Misuzu| Sonobe, Hiroshi| Hiroi, Makoto| Kubonishi, Ichiro| |
| 抄録 | <p>A new myeloid cell line, MTT-95, was established from the bone marrow of a patient with acute myelogenous leukemia (AML, M7). MTT-95 cells differentiate into mature basophilic cells in culture medium with no chemical component or cytokine. Surface phenotypes were as follows: CD11b 79.3%, CD13 92.4%, CD33 99.8%, CD34 87.9%, CD41a 77.6% and HLA-DR 0.3%. MTT-95 cells were strongly positive for glycoprotein IIb/IIIa by immunohistochemical staining and revealed metachromatic granules. MTT-95 cells seem to possess characteristics of both megakaryocytes and basophils. These findings suggest that MTT-95 cells are basophil progenitors. MTT-95 cells might be useful in the study not only of the biological aspects of basophils, but also of the diversities of AML (M7).</p> |
| キーワード | myeloid cell line acute myelogenous leukemia basophil megakaryocyte |
| Amo Type | Article |
| 発行日 | 1999-04 |
| 出版物タイトル | Acta Medica Okayama |
| 巻 | 53巻 |
| 号 | 2号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 95 |
| 終了ページ | 98 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | English |
| 論文のバージョン | publisher |
| 査読 | 有り |
| Web of Sience KeyUT | 000080058700006 |
| JaLCDOI | 10.18926/AMO/30761 |
|---|---|
| フルテキストURL | fulltext.pdf |
| 著者 | Mizobuchi, Noriko| Takahashi, Isao| Horimi, Tadashi| Yamamoto, Megumi| Hamada, Kyoko| Yorimitsu, Seiichi| Kubonishi, Ichiro| |
| 抄録 | <p>A new myeloid cell line, MTO-94, was established from the bone marrow of a patient with myelodysplastic syndrome (MDS). MTO-94 cells matured in culture medium without the addition of growth factors, and yielded neutrophils with pseudo-Pelger Huët anomaly or hypersegmentation until 6 months. Ten months after the start of cell cultivation, MTO-94 consisted of myeloblasts. Surface phenotypes were as follows: CD7 90.3%, CD13 99.6%, CD33 75.6%, HLA-DR 96.3% and CD34 0.9%. The karyotype was 46, XY, i(17q). The proliferation of MTO-94 cells was enhanced by rhlL-3, G-CSF, rhGM-CSF and rhSCF but not by rhlL-6 and erythropoietin. MTO-94 cells with i(17q) might be useful in the study of biological aspects of not only MDS, but also hematological malignancies with i(17q) as the sole chromosomal anomaly.</p> |
| キーワード | isochromosome 17q myeloid cell line myelodysplastic syndrome |
| Amo Type | Article |
| 発行日 | 1997-08 |
| 出版物タイトル | Acta Medica Okayama |
| 巻 | 51巻 |
| 号 | 4号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 227 |
| 終了ページ | 232 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | English |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 9284971 |
| Web of Sience KeyUT | A1997XU03200007 |