JaLCDOI 10.18926/AMO/32658
フルテキストURL fulltext.pdf
著者 Nakai, Hiromitsu| Hirakawa, Shuzo| Hayakawa, Nobuhiko| Amano, Tetsuki| Ota, Zensuke|
抄録 <p>Cytotoxic anti-thyroid microsomal autoantibodies are highly prevalent in sera of patients with Graves' disease, but in Graves' disease thyroid tissues rarely show destructive changes. We postulated that this might be due to membrane-associated complement regulatory proteins which protect target cells from injury by complement activation. We, therefore, investigated the expression of membrane attack complex inhibitory factor (MACIF) and decay accelerating factor (DAF) in the thyroid tissues from patients with Graves' disease, Hashimoto's thyroiditis, thyroid adenocarcinoma and normal human thyroid tissues. We found a high level of expression of MACIF and DAF in Graves' thyroid tissues. Using the membrane immunofluorescence and cell-ELISA techniques, we also investigated the factors which enhanced the MACIF and DAF expression in cultured thyroid cells. Thyroid stimulating hormone, phorbol 12, 13-dibutyrate and thyroid stimulating autoantibody enhanced the MACIF and DAF expression. These findings suggest that the membrane complement regulatory proteins increase in response to the thyroid stimulating factors such as thyroid stimulating autoantibody in Graves' disease and that this increase then protects the cells from damage due to complement activation by thyroid autoantibodies.</p>
キーワード MACIF DAF TSH phorbol 12 13-dibutyrate thyroid stimulating antibody
Amo Type Article
発行日 1992-10
出版物タイトル Acta Medica Okayama
46巻
5号
出版者 Okayama University Medical School
開始ページ 323
終了ページ 330
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1279944
Web of Sience KeyUT A1992JX49500002
JaLCDOI 10.18926/AMO/30383
フルテキストURL fulltext.pdf
著者 Tanimoto, Chikako| Hirakawa, Shuzo| Kawasaki, Hidetaka| Hayakawa, Nobuhiko| Ota, Zensuke|
抄録 <p>Etoposide (VP-16), one of the topoisomerase II (TopoII) inhibitors, interferes with TopoII by inducing the formation of and stabilizing a cleavable enzyme-DNA complex. VP-16 has been demonstrated to induce apoptosis in murine thymocytes. To clarify the mechanism of action of VP-16, we examined the in vitro effect of a non-cleavable-complex-forming type TopoII inhibitor, ICRF-193 which inhibits the DNA strand breakage induced by VP-16, on murine thymocytes in which apoptosis had been induced with VP-16. DNA fragmentation is characteristic of apoptosis. In the early stages, ICRF-193 decreased DNA fragmentation induced by VP-16, although this inhibitory effect decreased in the later. These data suggest that TopoII inhibitors induce apoptosis in murine thymocytes in two ways: with DNA-strand breaks in the early stage or without them. ICRF-193 itself induced apoptosis in murine thymocytes. The time course of DNA fragmentation caused by ICRF-193 was different from that of VP-16.</p>
キーワード etoposide thymocyte apoptosis ICRF-193 cleavable complex
Amo Type Article
発行日 1995-12
出版物タイトル Acta Medica Okayama
49巻
6号
出版者 Okayama University Medical School
開始ページ 281
終了ページ 286
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8770236
Web of Sience KeyUT A1995TM84600002