著者 Hatase, Osamu|
発行日 1969-08
出版物タイトル Acta Medicinae Okayama
23巻
4号
資料タイプ 学術雑誌論文
著者 Inaba, Kozo| Hatase, Osamu| Goto, Nobuyuki| Oda, Takuzo|
発行日 1969-08
出版物タイトル Acta Medicinae Okayama
23巻
4号
資料タイプ 学術雑誌論文
著者 Seki, Shuji| Hatase, Osamu| Oda, Takuzo|
発行日 1967-04
出版物タイトル Acta Medicinae Okayama
21巻
2号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/32414
フルテキストURL fulltext.pdf
著者 Matsui, Hideki| Kurosaki, Tomohiro| Tokuda, Masaaki| Hatase, Osamu|
抄録 <p>2-Mercaptoethanol increases the optical density of assay solutions at wavelengths between 280 to 400 nm, and therefore interferes with the measurement of protein concentration by the microbiuret method. Protein concentration can be determined in the presence of 2-mercaptoethanol up to 6 mM by modification of the method as follows: after the precipitation of protein by trichloroacetic acid in the presence of deoxycholate, the precipitate is resolubilized with NaOH solution. Dithiothreitol interfered with the protein determinations could by made in the presence of 4 mM of dithiothreitol with the modified microbiuret method. This modified method is time-saving and more reliable than other methods for protein determination, such as Lowry's method, in the presence of sulfhydryl reagents.</p>
キーワード microbiuret method sulfhydryl reagent protein determination
Amo Type Article
発行日 1983-04
出版物タイトル Acta Medica Okayama
37巻
2号
出版者 Okayama University Medical School
開始ページ 125
終了ページ 129
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6869062
Web of Sience KeyUT A1983QN63900004
JaLCDOI 10.18926/AMO/32071
フルテキストURL fulltext.pdf
著者 Takahashi, Fumio| Kuramitsu, Makoto| Tokuda, Masaaki| Matsui, Hideki| Itano, Toshifumi| Murakami, Tetsu-Hide| Hatase, Osamu| Nishida, Isamu|
抄録 <p>Cellular stimulating factors on cell proliferation in the supernatants of chick embryo carcases and adult muscles were studied. There were plural stimulating factors in embryonic and adult muscular supernatants that promoted cell proliferation without any supplement of sera and other materials. Salting-out methods with ammonium sulfate, ethanol fractionation, and isoelectric precipitation were used to isolate the stimulating factors, and these three methods proved the presence of plural stimulants on cell proliferation in the supernatants of chick embryo and adult muscles. The stimulants had altered physico-chemical properties and biological activities due to embryological development. The embryonic stimulants enhanced the synthesis of DNA and protein remarkably, and RNA synthesis in whole cell systems slightly. The muscular stimulants enhanced protein synthesis without any stimulation of DNA and RNA synthesis. Partial purification of the stimulants from the ethanol fractions was performed by DEAE-cellulose chromatography and Sephadex gel chromatography.</p>
キーワード chick growth factors cell proliferation growth regulation DNA and RNA synthesis protein synthesis
Amo Type Article
発行日 1979-06
出版物タイトル Acta Medica Okayama
33巻
3号
出版者 Okayama University Medical School
開始ページ 167
終了ページ 176
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 158945
JaLCDOI 10.18926/AMO/32056
フルテキストURL fulltext.pdf
著者 Hatase, Osamu| Fujii, Toshitake| Kuramitsu, Makoto| Itano, Toshifumi| Takahashi, Fumio| Murakami, Tetsuhide| Nishida, Isamu|
抄録 Factors that inhibit and stimulate cell proliferation were found to coexist in rat liver supernatant. The inhibitory and stimulatory factors were separated by ethanol fractionation. Both factors were sensitive to heat- and trypsin-treatment. The activity of the inhibitor was diminished by partial hepatectomy. The inhibitor from normal livers inhibited DNA and RNA synthesis in the L-cell system, but the same fraction from regenerating livers caused little or no inhibition of nucleic acid synthesis. The stimulatory factor from regenerating livers had a stronger effect on cell proliferation than that of normal livers. Furthermore, the inhibitor from normal livers depressed DNA synthesis in vivo in regenerating livers.
キーワード growth factors growth stimulants growth inhibitants rat liver cytoplasm cell proliferation
Amo Type Article
発行日 1979-04
出版物タイトル Acta Medica Okayama
33巻
2号
出版者 Okayama University Medical School
開始ページ 73
終了ページ 80
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 157671
JaLCDOI 10.18926/AMO/32055
フルテキストURL fulltext.pdf
著者 Tsutsui, Ken| Hatase, Osamu| Oda, Takuzo|
抄録 <p>Catalase was partially purified (about 380-fold purification) from the post-mitochondrial supernatant of bovine heart and compared with catalases from bovine erythrocytes and bovine liver. The electrophoretic mobility in polyacrylamide gel (pH 8.0) of heart catalase was the same as that of erythrocyte catalase and was smaller than that of the liver enzyme. The heart catalase was indistinguishable from erythrocyte catalase in regard to the molecular weights of subunit polypeptides, the inhibition patterns produced by several catalase inhibitors, and specific activity. The pH-activity curve of heart catalase consisted of a characteristic biphasic pattern with a peak at pH 7.5 and a shoulder at pH 10.</p>
キーワード catalse muscle bovine heart
Amo Type Article
発行日 1979-04
出版物タイトル Acta Medica Okayama
33巻
2号
出版者 Okayama University Medical School
開始ページ 103
終了ページ 111
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 157668
NAID 120002311382
JaLCDOI 10.18926/AMO/31596
フルテキストURL fulltext.pdf
著者 Miyamoto, Osamu| Itano, Toshifumi| Fujisawa, Mutsuo| Tokuda, Masaaki| Matsui, Hideki| Nagao, Seigo| Hatase, Osamu|
抄録 <p>Basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) were administered into the rat brain following unilateral fimbria-fornix transection. Both bFGF and NGF stimulated the sprouting of acetylcholinesterase (AChE) positive fibers in the hippocampus on the lesioned side. Furthermore, a small number of AChE-positive fibers were regenerated even when only the vehicle was administered. Rats treated with NGF as well as control group had only thin fibers, whereas those treated with bFGF had not only thin fibers but also thick fibers. These results indicate that intrinsic NGF is released and acts on damaged neurons directly, while bFGF acts them on directly and/or indirectly after brain injury.</p>
キーワード bFGF NGF regeneration acetylcholinesterase positive fibers sprouting
Amo Type Article
発行日 1993-06
出版物タイトル Acta Medica Okayama
47巻
3号
出版者 Okayama University Medical School
開始ページ 139
終了ページ 144
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8379341
Web of Sience KeyUT A1993LL12400001
著者 Hatase, Osamu| Tsutsui, Ken| Oda, Takuzo|
発行日 1976-06
出版物タイトル Acta Medica Okayama
30巻
3号
資料タイプ 学術雑誌論文
著者 Kanzaki, Yoshito| Hatase, Osamu| Ishii, Hideo| Oda, Takuzo|
発行日 1975-10
出版物タイトル Acta Medica Okayama
29巻
5号
資料タイプ 学術雑誌論文
著者 Takahashi, Fumio| Hatase, Osamu| Kuramitsu, Makoto| Itano, Toshifumi| Nisida, Isamu|
発行日 1977-02
出版物タイトル Acta Medica Okayama
31巻
1号
資料タイプ 学術雑誌論文
著者 Tsutsui, Ken| Koide, Noriko| Tomoda, Jun| Hayashi, Hideo| Hatase, Osamu| Oda, Takuzo|
発行日 1977-10
出版物タイトル Acta Medica Okayama
31巻
5号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/30708
フルテキストURL fulltext.pdf
著者 Kuramitsu, Makoto| Matsui, Hideki| Tokuda, Masaaki| Hatase, Osamu|
抄録 <p>Two factors from normal rat liver cytoplasm inhibited the proliferation of cultured L-929 fibroblasts. One was arginase, the other was a small molecular weight inhibitor stable to trypsin and heat treatment. The small molecular weight inhibitor inhibited the protein and DNA synthesis of L-cells. Inhibition of DNA synthesis was thought to be secondary to the inhibition of protein synthesis.</p>
キーワード cell proliferation growth factor inhibiting factor rat liver cytosol L-cells
Amo Type Article
発行日 1982-02
出版物タイトル Acta Medica Okayama
36巻
1号
出版者 Okayama University Medical School
開始ページ 1
終了ページ 10
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7064729
Web of Sience KeyUT A1982NE20000001
JaLCDOI 10.18926/AMO/30465
フルテキストURL fulltext.pdf
著者 Nakamura, Mitsuo| Itano, Toshifumi| Yamaguchi, Fuminori| Mizobuchi, Masayuki| Tokuda, Masaaki| Matsui, Hideki| Etoh, Siji| Hosokawa, Kiyoshi| Ohmoto, Takashi| Hatase, Osamu|
抄録 <p>Peptides and proteins in the extracellular space in the central nervous system were investigated in vivo using an intracerebral microdialysis probe. The molecular cut-off of the hollow fiber which was used for the probe was approximately 100 kDa. We examined recovery rates of several compounds in vitro. The recovery rates of proteins and peptides were between 7-28%, with the exceptions of substance P and insulin-like growth factor I. The recovery rates of monoamines and their metabolites were 22-40%. In in vivo studies, two major proteins with apparent molecular weights of 62 kDa and 12 kDa, and several minor proteins (28 kDa, 43 kDa, 52 kDa and 70 kDa) were detected by SDS-polyacrylamide gel electrophoresis in the dialysate from a probe implanted in the striatum of anesthetized rats. These results suggest that the newly developed, intracerebral microdialysis probe might be useful for investigating the dynamic changes of peptides and proteins in the central nervous system.</p>
キーワード protein peptide microdialysis extracellular space probe
Amo Type Article
発行日 1990-02
出版物タイトル Acta Medica Okayama
44巻
1号
出版者 Okayama University Medical School
開始ページ 1
終了ページ 8
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2330841
Web of Sience KeyUT A1990CT06800001