JaLCDOI 10.18926/AMO/31536
フルテキストURL fulltext.pdf
著者 Hashimoto, Kozo| Hattori, Teruhiko| Murakami, Kazuharu| Suemaru, Shouso| Kawada, Yoshiro| Ota, Zensuke|
抄録 <p>The stability of rat (human) CRF in serum, urine and tissue incubation medium was examined using Sephadex gel filtration and CRF radioimmunoassay with anti-rat (human) CRF serum. Human serum after incubation with rat (human) CRF for 1 h at 37 degrees C showed two peaks of CRF immunoreactivity on a Sephadex G-50 fine column. Most of the immunoreactivity coeluted with the rat (human) CRF marker. When rat (human) CRF was incubated with rat liver, kidney or hypothalamus, only 3.1-14.9% of the CRF was recovered at the rat (human) CRF position on gel filtration, and two to four CRF-immunoreactive peaks appeared after the rat (human) CRF marker. When rat (human) CRF was incubated with human urine (pH 6.0) for 24 h at room temperature, one peak of CRF immunoreactivity coeluted with the rat (human) CRF marker on Sephadex gel filtration. The urine extracts of normal rats showed some small peaks of CRF-like immunoreactivity on the Sephadex column, with the main peak appearing after authentic CRF. These results suggest that rat (human) CRF is relatively stable in serum and urine, but is easily degraded by tissue enzymes, with the degraded CRF fragments being excreted in the urine.</p>
キーワード rat(human) corticotropin releasing factor sephadex chromatography stability
Amo Type Article
発行日 1985-06
出版物タイトル Acta Medica Okayama
39巻
3号
出版者 Okayama University Medical School
開始ページ 199
終了ページ 206
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3875214
Web of Sience KeyUT A1985ALG3300005
JaLCDOI 10.18926/AMO/30349
フルテキストURL fulltext.pdf
著者 Hashimoto, Kozo| Murakami, Kazuharu| Hattori, Teruhiko| Ota, Zensuke|
抄録 <p>Plasma immunoreactive CRF measured by radioimmunoassay decreased rapidly after intravenous injection of synthetic ovine corticotropin releasing factor (CRF) and showed a bi-exponential decay curve in five macaca fuscatas. Half lives of plasma immunoreactive CRF were 5.8 +/- 1.4 (Mean +/- SEM) min for the fast component and 38.3 +/- 2.4 min for the slow component. A bolus injection of 5 micrograms/kg CRF significantly increased the plasma cortisol level. CRF at 5 micrograms/kg induced a delayed response of ACTH and cortisol. Arginine vasopressin (AVP) at 0.5 micrograms/kg induced a slight increase in plasma ACTH and cortisol, but AVP at 0.1 micrograms/kg evoked no significant increase. When 0.5 micrograms/kg CRF and 0.1 micrograms/kg AVP were administered simultaneously, significant ACTH and cortisol responses occurred. The results indicate that CRF and AVP act synergistically to stimulate ACTH secretion in vivo.</p>
キーワード corticotropin releasing factor arginine vasopressin adrenocorticotropin cortisol macaca fuscata
Amo Type Article
発行日 1984-06
出版物タイトル Acta Medica Okayama
38巻
3号
出版者 Okayama University Medical School
開始ページ 261
終了ページ 267
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6087620
Web of Sience KeyUT A1984SY25800006