JaLCDOI 10.18926/AMO/32629
フルテキストURL fulltext.pdf
著者 Morii, Kazuhiko| Shimomura, Hiroyuki| Nakagawa, Hiroshi| Hasui, Toshimi| Tsuji, Takao|
抄録 <p>Since detection of hepatitis C virus RNA by the polymerase chain reaction (PCR) showed that there existed anti-C100-3 (anti-HCV) antibody negative patients infected with HCV, we attempted to find out whether there were any clinical or viral genomic differences between the anti-HCV antibody positive and negative groups. One hundred and fifty-nine patients with chronic liver diseases with hepatitis C virus RNA in their sera were selected. Anti-HCV antibody was tested for anti-C100-3 antibody by an enzyme linked immunosorbent assay. The incidence of anti-HCV antibody was 129/159. The concentration of serum gamma-globulin, the titier of ZTT, and the positive rate of the PCR with the primers of the NS3/4 region (NS3/4PCR) were significantly higher in the anti-HCV antibody positive group than in the negative group. However, the other data such as alanine aminotransferase activity or past history were not significantly different. Nucleotide sequence of the cDNA fragments of NS3/4 region amplified by the PCR did not differ significantly between isolates from anti-HCV antibody positive and negative sera. The sequences observed in the present study did not differ significantly from those reported previously. Although there remains the possibility that the variation of viral genomic sequences may cause the absence of anti-HCV antibody, these results suggested that the individual clinical backgrounds or immunoreactivity of the patients might influence the antibody development.</p>
キーワード hepatitis C virus polymerase chain reaction anti-C100-3 antibody genetic variation
Amo Type Article
発行日 1992-08
出版物タイトル Acta Medica Okayama
46巻
4号
出版者 Okayama University Medical School
開始ページ 285
終了ページ 293
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1332424
Web of Sience KeyUT A1992JL44200009
JaLCDOI 10.18926/AMO/31583
フルテキストURL fulltext.pdf
著者 Nakagawa, Hiroshi| Shimomura, Hiroyuki| Hasui, Toshimi| Tsuji, Hideyuki| Tsuji, Takao|
抄録 <p>The negative strand RNA of hepatitis C virus, supposed to be a replicative intermediate of the virus appears to indicate viral replication. In this study, we detected the negative strand RNA by using reverse transcription-polymerase chain reaction with RNase A digestion to degrade the remaining positive strand genomic sequence of the virus after complementary DNA (cDNA) synthesis. In vitro transcribed positive-stranded mutant RNA was not detected by this method. Sample sera and liver tissues of 16 patients with chronic hepatitis C virus infection (liver fibrosis, 1; chronic hepatitis, 13; liver cirrhosis, 2) were analysed for negative strand RNA of hepatitis C virus. The negative strand RNA sequence was detected in 15 (93%) of 16 liver tissues and in 11 (78%) of 14 sera. The study demonstrated that negative strand RNA of hepatitis C virus in serum and liver tissue could be specifically detected.</p>
キーワード hepatitis C virus replication replicative intermediate polymerase chain reactuonM chronic liver disease
Amo Type Article
発行日 1993-10
出版物タイトル Acta Medica Okayama
47巻
5号
出版者 Okayama University Medical School
開始ページ 311
終了ページ 316
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8273455
Web of Sience KeyUT A1993ME47100004
JaLCDOI 10.18926/AMO/31549
フルテキストURL fulltext.pdf
著者 Hino, Naoki| Higashi, Toshihiro| Ueki, Tooru| Nakatsukasa, Harushige| Ooguchi, Souhei| Ashida, Kouzou| Fujio, Kouzou| Takaki, Akinobu| Yonei, Taiji| Hasui, Toshimi| Tsuji, Hideyuki| Tsuji, Takao|
抄録 <p>We report here a case of hepatocellular carcinoma (HCC) with multiple lung metastases, which were disappeared by treatment of OK-432. The patient was a 65-year-old man and was diagnosed in 1986 with a small (17 x 11 mm) HCC in the anterior-superior segment of the liver. A part of the right hepatic lobe including the tumor was surgically removed, and transarterial injections of adriamycin (10 mg/week) and subcutaneous injections of OK-432 (10 KE/week) were given. Two and a half years later, recurrence of HCC in the liver and its invasion to vena cava inferior (IVC) were found. OK-432 administration was then stopped and percutaneous ethanol injection therapy (PEIT) was performed 10 times. Six months later, the PEIT was effective and the liver tumor with IVC invasion diminished. However, multiple lung metastases were visible on roentgenograms of the chest, and serum alphafetoprotein (AFP) concentration increased to 50,000 ng/ml. The OK-432 treatment resumed. After 6 months of OK-432 treatment, the multiple lung metastases were disappeared and the serum AFP level decreased to 100 ng/ml. At present, the patient is surviving without any sign of recurrence in either the liver or the lung. The clinical course of this case suggests that OK-432 might have effectively treated lung metastases of HCC, although the exact mechanisms are at present unclear.</p>
キーワード hepatocellular carcinoma OK-432 pulmonary metastasis
Amo Type Article
発行日 1993-08
出版物タイトル Acta Medica Okayama
47巻
4号
出版者 Okayama University Medical School
開始ページ 289
終了ページ 292
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7692705
Web of Sience KeyUT A1993LV73800011
JaLCDOI 10.18926/AMO/31311
フルテキストURL fulltext.pdf
著者 Tsuji, Hideyuki| Shimomura, Hiroyuki| Fujio, Kozo| Wato, Masaki| Kondo, Junichi| Hasui, Toshimi| Ishi, Yasushi| Fujioka, Shin-ichi| Tsuji, Takao|
抄録 <p>To evaluate viral interference between hepatitis B and C, we studied coinfected patients serologically and molecular biologically. Twenty-seven patients positive for hepatitis B surface antigen (HBsAg) and anti-hepatitis C virus (HCV) antibody, were classified into Groups BC-L and BC-H according to DNA-polymerase activity (less or greater than 100 cpm, respectively). Patients with hepatitis B or C alone were also enrolled as controls. HCV-RNA was detected more often in Group BC-L than in Group BC-H. Genotype 1b of HCV was determined in 75% of Group BC-H, 87.5% of Group BC-L, and 70.7% of hepatitis C-only patients. Activity of DNA-polymerase in coinfected patients was lower in patients positive for HCV-RNA as compared with those negative. HBsAg titers tended to be lower in coinfected patients than in patients with hepatitis B virus (HBV) alone. In conclusion, in coinfection, HBV may suppress the replication of HCV and HCV appears to reduce the expression of HBsAg and probably suppresses HBV replication.&#60;/P&#62;</p>
キーワード hepatitis B virus hepatitis C virus double infection hepatitis B surface antigen hepatitis C virusRNA
Amo Type Article
発行日 1998-04
出版物タイトル Acta Medica Okayama
52巻
2号
出版者 Okayama University Medical School
開始ページ 113
終了ページ 118
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9588227
Web of Sience KeyUT 000073363000007
JaLCDOI 10.18926/AMO/31121
フルテキストURL fulltext.pdf
著者 Hasui, Toshimi| Shimomura, Hiroyuki| Tsuji, Hideyuki| Wato, Masaki| Tsuji, Takao|
抄録 <p>Recently, factors predicting the response to interferon (IFN) therapy against hepatitis C virus (HCV) have received much attention. To evaluate the usefulness of the quantitation of intrahepatic HCV RNA as a predictive marker of the response to IFN therapy, we compared the amount of intrahepatic HCV RNA with serum levels in 16 patients. Eleven patients who had 10(10) copies/g or more of intrahepatic HCV RNA had increased level of serum alanine aminotransferase (ALT) after IFN therapy, while 4 of 5 patients who had less than 10(10) copies/g of intrahepatic HCV RNA achieved sustained normalization of serum ALT level and were designated as complete responders. Four complete responders possessed significantly less HCV RNA in the liver parenchyma than partial and nonresponders (P = 0.010, Mann-Whitney U-test), but the amount of HCV RNA in the serum was not significantly different between those groups. In conclusion, the results suggest that the quantitation of intrahepatic HCV RNA is a better indicator of the response to IFN therapy than serum HCV RNA.</p>
キーワード hepatitis C virus interferon liver tissue quantitation polymerase chain reaction
Amo Type Article
発行日 1994-06
出版物タイトル Acta Medica Okayama
48巻
3号
出版者 Okayama University Medical School
開始ページ 151
終了ページ 157
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7524269
Web of Sience KeyUT A1994NV04300006