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ID 32972
フルテキストURL
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著者
Imai, Akihiro Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Hanzawa, Yoshie Division of Biological Sciences, Graduate School of Science, Hokkaido University
Komura, Mio Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Yamamoto, Kotaro T. Division of Biological Sciences, Graduate School of Science, Hokkaido University
Komeda, Yoshibumi Division of Biological Sciences, Graduate School of Science, Hokkaido University
Takahashi, Taku Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
抄録
Loss-of-function mutants of the Arabidopsis thaliana ACAULIS 5 (ACL5) gene, which encodes spermine synthase, exhibit a severe dwarf phenotype. To elucidate the ACL5-mediated regulatory pathways of stem internocle elongation, we isolated four suppressor of acaulis (sac) mutants that reverse the acl5 dwarf phenotype. Because these mutants do not rescue the dwarfism of known phytohormone-related mutants, the SAC genes appear to act specifically on the ACL5 pathways. We identify the gene responsible for the dominant sac51-d mutant, which almost completely suppresses the acl5 phenotype. sac51-d disrupts a short upstream open reading frame (uORF) of SAC51, which encodes a bHLH-type transcription factor. Our results indicate that premature termination of the uORF in sac51-d results in an increase in its own transcript level, probably as a result of an increased translation of the main ORF. We suggest a model in which ACL5 plays a role in the translational activation of SAC51, which may lead to the expression of a subset of genes required for stem elongation.
キーワード
Arabidopsis thaliana
Polyamine
Spermine
Stem elongation
Upstream ORF
備考
Digital Object Identifer:10.1242/dev.02535
Published with permission from the copyright holder. This is the institute's copy, as published in Development, Volume 133, Issue 18, September 2006, Pages 3575-3585.
Publisher URL:http://dx.doi.org/10.1242/dev.02535
Direct access to Thomson Web of Science record
Copyright © 2006 The Company of Biologists Ltd. All rights reserved.
発行日
2006-09-15
出版物タイトル
Development
133巻
18号
出版者
The Company of Biologists
開始ページ
3575
終了ページ
3585
ISSN
0950-1991
NCID
AA10667805
資料タイプ
学術雑誌論文
言語
English
著作権者
The Company of Biologists
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT
Submission Path
biology_general/6