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ID 31747
JaLCDOI
フルテキストURL
著者
Wakiguchi, Hiroshi Kochi Medical School
Fujieda, Mikiya Kochi Medical School
Matsumoto, Kenji Kochi Medical School
Ohara, Yuji Kochi Medical School
Kuroiwa, Yoshio Kochi Medical School
Wakiguchi, Akiko Okayama University
Shiraishi, Taisuke Okayama University
Oda, Megumi Okayama University
Kurashige, Takanobu Okayama University
Kitamura, Isamu Okayama University
抄録

Antibody activity, especially that involved in the reaction of antibody-dependent cell-mediated cytotoxicity (ADCC), of five commercially available human gammaglobulin preparations (standard, pepsin-treated, plasmin-treated, polyethylene glycol-fractionated and S-sulfonated gammaglobulin) was measured. All these gammaglobulin preparations had high titers of hemagglutination inhibition and neutralizing antibody against measles virus. In ADCC reaction, the pepsin-treated gammaglobulin preparation showed no antibody activity. The standard gammaglobulin preparation showed weak activity only when highly diluted. The remaining three preparations showed high activity. Though the S-sulfonated gammaglobulin preparation showed no activity in ADCC reaction, it showed high activity after reconversion by means of oxidation and reduction in vitro. The plasmin-treated gammaglobulin preparation showed greater activity than the polyethylene glycol-fractionated preparation of the optimal concentration. In ADCC tests using the plasmin-treated gammaglobulin preparation, K cell activity was strongly inhibited by Hg (thimerosal), while, in those using the standard gammaglobulin preparation, the activity was hardly influenced by Hg, suggesting that the low ADCC activity of the standard gammaglobulin preparation of high concentrations was due to the inhibitory effect of aggregated immunoglobulin G molecules.

キーワード
antibody-dependent cell-mediated cytotoxicity
measles
immunology
gammaglobulin preparation
Amo Type
Article
発行日
1987-04
出版物タイトル
Acta Medica Okayama
41巻
2号
出版者
Okayama University Medical School
開始ページ
71
終了ページ
79
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Science KeyUT