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ID 30558
JaLCDOI
フルテキストURL
Thumnail fulltext.pdf 1.57 MB
著者
Yamada, Gotaro Okayama University
Takahashi, Takashi Kitasato Institute
Mizuno, Motowo Okayama University
Sakamoto, Yuji Okayama University
Kaga, Kazumi Kitasato Imstitute
Kobayashi, Toshinari Kawasaki Medical College
Nagashima, Hideo Okayama University
抄録

A recently modified method using peroxidase labeled antibodies for light and electron microscopic demonstration of hepatitis B virus (HBV) was applied to the evaluation of hepatitis B surface antigen (HBsAg) on the surface of liver cells in biopsy specimens from 24HBsAg chronic carriers. Membranous distribution of HBsAg was demonstrated in diffuse or scattered hepatocytes in all 4 asymptomatic carriers and in 3 of the 20 patients with HBsAg-positive chronic active hepatitis or liver cirrhosis. In these patients with membranous expression of HBsAg, hepatitis B e antigen, Dane particles and DNA polymerase were often detected in sera, and large amounts of hepatitis B core antigen appeared in the liver. These results suggest that membrane-bound HBsAg may be expressed by the HBV genome. The ultrastructural study of liver cells showing membranous expression disclosed dense deposits of reaction product indicative of HBsAg on the cell membrane and/or on assembled particles within the extracellular space. In some hepatocytes showing both diffuse cytoplasmic and membranous expression of HBsAg, HBsAg-positive membrane of cisternae open to the intercellular space was connected with the liver cell membrane. These findings supported the conjecture that HBV associated antigens are integrated into the liver cell membrane.

キーワード
type B hepatitis
membrane-bound HBsAg
immunoelectron microscopy
peroxidase-labeled antibody method.
Amo Type
Article
発行日
1980-06
出版物タイトル
Acta Medica Okayama
34巻
3号
出版者
Okayama University Medical School
開始ページ
175
終了ページ
187
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT