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ID 1
Eprint ID
1
フルテキストURL
タイトル(別表記)
Genetic Analysis, Expression in Eschericia coli of Aconitase from Chemolithotrophic Acidithiobacillus thiooxidans
著者
金原 陽平 岡山大学
田村 隆 岡山大学
徳田 千束 オリエンタル酵母工業(株)
中村 淳雄 オリエンタル酵母工業(株)
松川 寛和 オリエンタル酵母工業(株)
稲垣 賢二 岡山大学
抄録
An aconitase from Acidithiobacillus thiooxidans was purified and characterized, and its gene was cloned. The cloned aconitase gene (acn) was expressed in Escherichia coli JM 109; aconitase activity was found in the cell extarct. The acn gene encodes a 646-amino acid polypeptide and is located upstream of the isocitrate dehydrogenase gene (icd). A. thiooxidans aconitase showes high sequence similar to pig heart aconitase and E.coli aconitase B. Twenty-five of twenty-seven active site residues assigned in pig heart aconitase are conserved in A. thiooxidans aconitase. The enzyme was purified by DEAE-Toyopearl 650M column chromatogrophy. The purified enzyme had an optimum pH of 7.5 and an optimum temperature of 60 C. Thermal inactivation studies of the purified enzyme revealed the enzyme activity to be uninfluenced after one hour incubation at 40 c. Enzyme activity was retained 100% after incubation of the enzyme at pH 6.0-9.0 for 60min. The A. thiooxidans aconitase was composed of a single polypeptide chain with a molecular mass of 66 kDa.
キーワード
Aconitase
Acidithiobacillus
Isocitrate dehydrogenase
発行日
2005-02-01
出版物タイトル
岡山大学農学部学術報告
出版物タイトル(別表記)
Scientific reports of the Faculty of Agriculture, Okayama University
94巻
1号
出版者
岡山大学農学部
出版者(別表記)
Faculty of Agriculture,Okayama University
開始ページ
1
終了ページ
7
ISSN
0474-0254
NCID
AN00033029
資料タイプ
紀要論文
言語
Japanese
論文のバージョン
publisher
査読
無し
Eprints Journal Name
srfa