このエントリーをはてなブックマークに追加
ID 34246
フルテキストURL
Thumnail fulltext.pdf 5.35 MB
著者
Sawada, Keisuke Okayama University
Echigo, Noriko Okayama University
Juge, Narinobu Okayama University
Miyaji, Takaaki Okayama University
Otsuka, Masato Okayama University
Omote, Hiroshi Okayama University
Yamamoto, Akitsugu Nagahama Institute of Technology
Moriyama, Yoshinori Okayama University
抄録

ATP is a major chemical transmitter in purinergic signal transmission. Before secretion, ATP is stored in secretory vesicles found in purinergic cells. Although the presence of active transport mechanisms for ATP has been postulated for a long time, the proteins responsible for its vesicular accumulation remains unknown. The transporter encoded by the human and mouse SLC17A9 gene, a novel member of an anion transporter family, was predominantly expressed in the brain and adrenal gland. The mouse and bovine counterparts were associated with adrenal chromaffin granules. Proteoliposomes containing purified transporter actively took up ATP, ADP, and GTP by using membrane potential as the driving force. The uptake properties of the reconstituted transporter were similar to that of the ATP uptake by synaptic vesicles and chromaffin granules. Suppression of endogenous SLC17A9 expression in PC12 cells decreased exocytosis of ATP. These findings strongly suggest that SLC17A9 protein is a vesicular nucleotide transporter and should lead to the elucidation of the molecular mechanism of ATP secretion in purinergic signal transmission.

キーワード
chromaffin granule
synaptic vesicle
ATP
storage and exocytosis
purinergic signaling.
備考
Published with permission from the copyright holder.
This is a author's copy,as published in PNAS , 2008
Publisher URL: http://dx.doi.org/10.1073/pnas.0800141105
Copyright © 2008 by the National Academy of Sciences
発行日
2008-04-01
出版物タイトル
Proceedings of the National Academy of Sciences of the United States of America
105巻
15号
開始ページ
5683
終了ページ
5686
資料タイプ
学術雑誌論文
言語
English
査読
有り
DOI
Submission Path
biochemistry/8