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ID 53419
フルテキストURL
著者
Miyaji, Takaaki Advanced Science Research Center, Okayama University
Kuromori, Takashi Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science
Takeuchi, Yu Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Yamaji, Naoki Institute of Plant Science and Resources, Okayama University
Yokosho, Kengo Institute of Plant Science and Resources, Okayama University
Shimazawa, Atsushi Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sugimoto, Eriko Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science
Omote, Hiroshi Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ma, Jian Feng Institute of Plant Science and Resources, Okayama University
Shinozaki, Kazuo Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science
Moriyama, Yoshinori Advanced Science Research Center, Okayama University
抄録
Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4;4 protein exhibit membrane potential- and Cl-dependent ascorbate uptake. The AtPHT4;4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4;4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4;4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress.
発行日
2015-01-05
出版物タイトル
Nature Communications
6巻
出版者
Nature Publishing Group
ISSN
2041-1723
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1038/ncomms6928
言語
English
著作権者
© 2015 Macmillan Publishers Limited. All rights reserved. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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