start-ver=1.4
cd-journal=joma
no-vol=66
cd-vols=
no-issue=3
article-no=
start-page=253
end-page=261
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2012
dt-pub=201206
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Clostridium botulinum Type E Toxins Bind to Caco-2 Cells by a Different Mechanism from That of Type A Toxins
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Cultured Clostridium botulinum strains produce progenitor toxins designated as 12S, 16S, and 19S toxins. The 12S toxin consists of a neurotoxin (NTX, 7S) and a non-toxic non-hemagglutinin (NTNH). The 16S and 19S toxins are formed by conjugation of the 12S toxin with hemagglutinin (HA), and the 19S toxin is a dimer of the 16S toxin. Type A cultures produce all 3 of these progenitor toxins, while type E produces only the 12S toxin. The 7S toxin is cleaved into heavy (H) and light (L) chains by a protease(s) in some strains, and the H chain has 2 domains, the N-terminus (Hn) and C-terminus (Hc). It has been reported that type A toxins bind to the intestinal cells or cultured cells via either HA or Hc. In this study, we investigated the binding of type A and E toxins to Caco-2 cells using Western blot analysis. Both the type E 7S and 12S toxins bound to the cells, with the 7S toxin binding more strongly, whereas, in the type A strain, only the 16S/19S toxins showed obvious binding. Pre-incubation of the type E 7S toxin with IgG against recombinant type E Hc significantly inhibited the 7S toxin binding, indicating that Hc might be a main binding domain of the type E toxin.
en-copyright=
kn-copyright=

en-aut-name=ZhangKai
en-aut-sei=Zhang
en-aut-mei=Kai
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=YamamotoYumiko
en-aut-sei=Yamamoto
en-aut-mei=Yumiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SuzukiTomonori
en-aut-sei=Suzuki
en-aut-mei=Tomonori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=YokotaKenji
en-aut-sei=Yokota
en-aut-mei=Kenji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=MaShaobo
en-aut-sei=Ma
en-aut-mei=Shaobo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=Nengah Dwi  FatmawatiNi
en-aut-sei=Nengah Dwi  Fatmawati
en-aut-mei=Ni
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=OgumaKeiji
en-aut-sei=Oguma
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine

affil-num=2
en-affil=
kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine

affil-num=3
en-affil=
kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine

affil-num=4
en-affil=
kn-affil=Graduate School of Health Sciences, Okayama University

affil-num=5
en-affil=
kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine

affil-num=6
en-affil=
kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine

affil-num=7
en-affil=
kn-affil=Department of Bacteriology, Okayama University Graduate School of Medicine
en-keyword=Clostridum botulinum
kn-keyword=Clostridum botulinum
en-keyword=neurotoxins
kn-keyword=neurotoxins
en-keyword=Caco-2
kn-keyword=Caco-2
en-keyword=binding
kn-keyword=binding
en-keyword=Hc
kn-keyword=Hc
END