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ID 48821
フルテキストURL
著者
Yamasaki, Akinori Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine
Menon, Shekar Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine
Yu, Sidney Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine
Barrowman, Jemima Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine
Meerloo, Timo Department of Cellular and Molecular Medicine, University of California at San Diego
Oorschot, Viola Cell Microscopy Center, Department of Cell Biology, University Medical Center Utrecht
Klumperman, Judith Cell Microscopy Center, Department of Cell Biology, University Medical Center Utrecht
Satoh, Ayano Department of Cell Biology, Yale University School of Medicine 科研費研究者番号
Ferro-Novick, Susan Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine
抄録
The GTPase Rab1 regulates endoplasmic reticulum-Golgi and early Golgi traffic. The guanine nucleotide exchange factor (GEF) or factors that activate Rab1 at these stages of the secretory pathway are currently unknown. Trs130p is a subunit of the yeast TRAPPII (transport protein particle II) complex, a multisubunit tethering complex that is a GEF for the Rab1 homologue Ypt1p. Here, we show that mammalian Trs130 (mTrs130) is a component of an analogous TRAPP complex in mammalian cells, and we describe for the first time the role that this complex plays in membrane traffic. mTRAPPII is enriched on COPI (Coat Protein I)-coated vesicles and buds, but not Golgi cisternae, and it specifically activates Rab1. In addition, we find that mTRAPPII binds to γ1COP, a COPI coat adaptor subunit. The depletion of mTrs130 by short hairpin RNA leads to an increase of vesicles in the vicinity of the Golgi and the accumulation of cargo in an early Golgi compartment. We propose that mTRAPPII is a Rab1 GEF that tethers COPI-coated vesicles to early Golgi membranes.
発行日
2009-10-01
出版物タイトル
Molecular Biology of the Cell
20巻
19号
出版者
The American Society for Cell Biology
開始ページ
4205
終了ページ
4215
ISSN
1059-1524
NCID
AA12024500
資料タイプ
学術雑誌論文
プロジェクト
異分野融合先端研究コア
オフィシャル URL
http://www.molbiolcell.org/content/20/19/4205.full
言語
English
著作権者
© 2009 by The American Society for Cell Biology
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publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT