JaLCDOI 10.18926/AMO/30338
フルテキストURL fulltext.pdf
著者 Minami, Junzabro| Okabe, Akinobu| Nagata, Akihide| Hayashi, Hideo|
抄録 <p>A modified method of passive immune hemolysis (PIH) was applied to the quantitative assay of heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli. The method enabled the measurement of 0.2 to 1.2 ng LT. The production of LT by enterotoxigenic E. coli under various conditions was analyzed using the modified method. LT production was intense during the logarithmic growth phase and decreased during the stationary growth phase. Lincomycin (50 to 100 micrograms/ml) affected cell growth slightly, but enhanced production of LT until the late-stationary growth phase. About 90% of the LT produced was retained in the cell, and the rest was excreted into the culture medium. The initial pH of the culture medium affected LT production. Alkaline pH enhanced LT production, though growth was depressed. Aeration enhanced both growth and LT production.</p>
キーワード heat-labile enterotoxin E coli passive immune hemolysis
Amo Type Article
発行日 1984-10
出版物タイトル Acta Medica Okayama
出版者 Okayama University Medical School
開始ページ 461
終了ページ 469
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6393717
Web of Sience KeyUT A1984TQ61100006