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ID 30338
JaLCDOI
フルテキストURL
著者
Minami, Junzabro Kagawa Medical School
Okabe, Akinobu Kagawa Medical School
Nagata, Akihide Okayama Univeristy
Hayashi, Hideo Kagawa Medical School
抄録

A modified method of passive immune hemolysis (PIH) was applied to the quantitative assay of heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli. The method enabled the measurement of 0.2 to 1.2 ng LT. The production of LT by enterotoxigenic E. coli under various conditions was analyzed using the modified method. LT production was intense during the logarithmic growth phase and decreased during the stationary growth phase. Lincomycin (50 to 100 micrograms/ml) affected cell growth slightly, but enhanced production of LT until the late-stationary growth phase. About 90% of the LT produced was retained in the cell, and the rest was excreted into the culture medium. The initial pH of the culture medium affected LT production. Alkaline pH enhanced LT production, though growth was depressed. Aeration enhanced both growth and LT production.

キーワード
heat-labile enterotoxin
E
coli
passive immune hemolysis
Amo Type
Article
発行日
1984-10
出版物タイトル
Acta Medica Okayama
38巻
5号
出版者
Okayama University Medical School
開始ページ
461
終了ページ
469
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT