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ID 53976
フルテキストURL
著者
Nakagawa, Tomoyuki Faculty of Applied Biological Science, Gifu University
Mitsui, Ryoji Department of Biochemistry, Faculty of Science, Okayama University of Science
Tani, Akio Institute of Plant Science and Resources, Okayama University
Sasa, Kentaro Faculty of Applied Biological Science, Gifu University
Tashiro, Shinya Faculty of Applied Biological Science, Gifu University
Iwama, Tomonori Faculty of Applied Biological Science, Gifu University
Hayakawa, Takashi Faculty of Applied Biological Science, Gifu University
Kawai, Keiichi Tokai Gakuin University
抄録
In the methylotrophic bacterium Methylobacterium extorquens strain AM1, MxaF, a Ca2+-dependent methanol dehydrogenase (MDH), is the main enzyme catalyzing methanol oxidation during growth on methanol. The genome of strain AM1 contains another MDH gene homologue, xoxF1, whose function in methanol metabolism has remained unclear. In this work, we show that XoxF1 also functions as an MDH and is La3+-dependent. Despite the absence of Ca2+ in the medium strain AM1 was able to grow on methanol in the presence of La3+. Addition of La3+ increased MDH activity but the addition had no effect on mxaF or xoxF1 expression level. We purified MDH from strain AM1 grown on methanol in the presence of La3+, and its N-terminal amino acid sequence corresponded to that of XoxF1. The enzyme contained La3+ as a cofactor. The ΔmxaF mutant strain could not grow on methanol in the presence of Ca2+, but was able to grow after supplementation with La3+. Taken together, these results show that XoxF1 participates in methanol metabolism as a La3+-dependent MDH in strain AM1.
備考
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
発行日
2013-03-22
出版物タイトル
PLOS ONE
7巻
11号
出版者
PUBLIC LIBRARY SCIENCE
開始ページ
e50480
ISSN
1932-6203
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1371/journal.pone.0050480
言語
English
著作権者
© 2012 Nakagawa et al.
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT