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ID 32293
JaLCDOI
フルテキストURL
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著者
Jin, Zaishun Okayama University
Teramoto, Norihiro Okayama University
Yoshino, Tadashi Okayama University
Takada, Kenzo Hokkaido University
Oka, Takashi Okayama University
Hayashi, Kazuhiko Okayama University
Akagi, Tadaatsu Okayama University
抄録

It has been reported that Epstein-Barr virus (EBV) resides in resting B cells in vivo. However, an ideal in vitro system for studying EBV latent infection in vivo has not yet been established. In this study, a mantle cell lymphoma line, SP53, was successfully infected with a recombinant EBV containing a neomycin-resistant gene. The EBV-carrying SP53 cells were obtained by selection using G418. They expressed EBER-1, EBNAs, and LMP1; this expression pattern of the EBV genes was similar to that in a lymphoblastoid cell line (LCL). However, proliferation assay showed that the EBV-carrying SP53 cells have a doubling time of 73 h, compared with 57 h of SP53 cells. Transplantation of 10(8) SP53 cells to nude mice formed tumors in 4 of 10 mice inoculated, but the EBV-carrying SP53 cells did not. Unexpectedly, EBV infection reduced the proliferation and tumorigenicity of SP53 cells. However, the EBV-carrying SP53 cells showed higher resistance to apoptosis induced by serum starvation than did the SP53 cells. The inhibition of proliferation and the resistance to apoptosis induced in SP53 cells by EBV infection indicate that this cell line might to some extent provide a model of in vivo EBV reservoir cells.

キーワード
Epstein-Barr virus
mantle cell lymphoma
latent infection
in vivo reservoir
SP53 line
Amo Type
Article
発行日
2000-10
出版物タイトル
Acta Medica Okayama
54巻
5号
出版者
Okayama University Medical School
開始ページ
193
終了ページ
200
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT