JaLCDOI 10.18926/AMO/30507
フルテキストURL fulltext.pdf
著者 Ogata, Masatoshi| Taga, Hiroko| Taketa, Kazuhisa|
抄録 <p>&#60;P&#62;Microdetermination of alpha-fetoprotein (AFP) glycoforms by lectin affinity electrophoresis followed by chemiluminescence reaction using horseradish peroxidase (POD) or alkaline phosphatase (ALP) in antibody-affinity blotting was developed. The intensity of chemiluminescence obtained by ALP was greater than that by POD; however, the coefficient of variation with POD was less than that with ALP. The optimized sensitivity of the chemiluminescence method with POD was two times that of the most sensitive colorimetric method currently available in terms of the chemiluminescence intensity per unit AFP concentration. The lower detection limit by the chemiluminescence method with POD (0.5 ng/ml) was much lower than that by the colorimetric method (3 ng/ml). Both methods gave identical percentages of lentil lectin- and erythroagglutinating phytohemagglutinin-reactive minor bands using a serum with 52 ng/ml AFP. This result indicates that microdetermination of AFP glycoforms by chemiluminescence after lectin-affinity electrophoresis was more sensitive than currently available methods and that it is potentially useful for clinical application&#60;/P&#62;</p>
キーワード alpha-fetoprotein lectin affinity electrophoresis chemiluminescence antibode-affinity biotting avidin-biotin complex
Amo Type Article
発行日 1996-06
出版物タイトル Acta Medica Okayama
出版者 Okayama University Medical School
開始ページ 119
終了ページ 124
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8805850
Web of Science KeyUT A1996UU60400001